DNA cloning and assembly : methods and protocols / edited by Sunil Chandran, Kevin W. George. -- New York, NY : Humana Press, 2020. – (58.17 /M592 /v.2205)

Contents

PART I  IN SILICO TOOLS FOR DNA DESIGN AND ASSEMBLY

1  An Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology

2  Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5

3  The AssemblX Toolkit for Reliable and User-Friendly Multigene Assemblies

PART II  HIGH THROUGHPUT WORKFLOWS

4  High-Throughput PCR for DNA Part Generation

5  High-Throughput DNA Assembly Using Yeast Homologous Recombination

6  Highly Multiplexed, Semiautomated Nextera Next-Generation Sequencing (NGS) Library Preparation

PART III  DNA ASSEMBLY STANDARDS

7  Generation of MoClo Standard Parts Using Golden Gate Cloning

8  Assembly of Multigene Constructs Using the Modular Cloning System MoClo

9  Hierarchical Modular DNA Assembly Using MetClo

10  PaperClip DNA Assembly: Reduce, Reuse, Recycle

11  Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plants

12  Mobius Assembly

13  Design and Implementation of Multi-protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly

14  BASIC: A Simple and Accurate Modular DNA Assembly Method

15  Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Host

PART IV  COMBINATORIAL METHODS FOR PATHWAY OPTIMIZATION

16  A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly

17  SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast

Index