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Coronaviruses : methods and protocols / edited by Helena J. Maier, Erica Bickerton. -- 2nd ed. -- New York, NY : Humana Press, 2020. – (58.17 /M592 /v.2203) |
Contents
Preface
Contributors
1
Coronaviruses: An Updated Overview of Their Replication and Pathogenesis
PART I
CORONAVIRUS DETECTION, DISCOVERY, AND EVOLUTION
2
Discovery of Novel Coronaviruses in Rodents
3
Detection and Discovery of Coronaviruses in Wild Bird Populations
4
Competitive ELISA for the Detection of Serum Antibodies Specific for
Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
5
Whole-Genome Sequencing Protocols for IBV and Other Coronaviruses Using
High-Throughput Sequencing
PART II
PROPAGATION AND TITRATION OF CORONAVIRUSES
6
Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A
Case Study
7 The
Preparation of Chicken Kidney Cell Cultures for Virus Propagation
8 The
Preparation of Chicken Tracheal Organ Cultures and Their Application for
Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation
9
Isolation and Propagation of Coronaviruses in Embryonated Eggs
10
Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures
11
Quantification of Coronaviruses by Titration In Vitro and Ex Vivo
PART III
MANIPULATING THE GENOMES OF CORONAVIRUSES
12
Transient Dominant Selection for the Modification and Generation of
Recombinant Infectious Bronchitis Coronaviruses
13
In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a
Synthetic Genomics Pipeline
PART IV
STUDYING VIRUS-HOST INTERACTIONS
14
Proximity Labeling for the Identification of Coronavirus-Host Protein
Interactions
15
Using Yeast to Identify Coronavirus-Host Protein Interactions..
16
Determining How Coronaviruses Overcome the Interferon and Innate Immune
Response
17 Ribopuromycylation
in Coronavirus-Infected Cells
PART V
IMAGING CORONAVIRUS INFECTIONS
18
Visualizing Coronavirus Entry into Cells
19
Preparation of Cultured Cells Using High-Pressure Freezing and Freeze
Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron
Microscope
Index
