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Channelrhodopsin : methods and protocols / edited by Robert E. Dempski. -- New York, NY : Humana Press, 2021. – (58.17 /M592 /v.2191) |
Contents
Preface
PART I
BASIC SCIENCE
1
Molecular Dynamics Simulations of Channelrhodopsin Chimera, C1C2
2
Computing Potential of the Mean For& Profiles for Ion Permeation
Through Channelrhodopsin Chimera, C1C2
3
Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in
Pichia pastoris for Biophysical Investigations
4
Characterizing Channelrhodopsin Channel Properties Via Two-Electrode
Voltage Clamp and Kinetic Modeling
PART II
CELLS
5
Charge Transport by Light-Activated Rhodopsins Determined by
Electrophysiological Recordings
6
Probing Channelrhodopsin Electrical Activity in Algal Cell Populations
7
Two-Photon Optogenetic Stimulation of Drosophila Neurons
8
Probing Synaptic Signaling with Optogenetic Stimulation and Genetically
Encoded Calcium Reporters
9
Optogenetics to Interrogate Neuron-Glia Interactions in Pups and Adults
10
Chronic Optogenetic Pacing of Human-Induced Pluripotent Stem
Cell-Derived Engineered Cardiac Tissues
PART III ANIMALS
11
Patterned Optogenetic Stimulation Using a DMD Projector
12
Selecting Channelrhodopsin Constructs for Optimal Visual Restoration in
Differing Light Conditions
13
Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses
from Larval Zebrafish
14
Automated Functional Screening for Modulators of Optogenetically
Activated Neural Responses in Living Organisms
15
Optogenetic Interrogation of ChR2-Expressing GABAergic Interneurons
After Transplantation into the Mouse Brain
16
Application of Targeting-Optimized Chronos for Stimulation of the
Auditory Pathway
17
Channelrhodopsins for Cell-Type Specific Illumination of Cardiac
Electrophysiology
18
Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice
19
Dissecting Mechanisms of Motivation within the Nucleus Accumbens Using
Optogenetics
20
Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin
21
Optical Manipulation of Perfused Mouse Heart Expressing
Channelrhodopsin-2 in Rhythm Control
22
Chronic Optogenetic Stimulation in Freely Moving Rodents
Index
