Methods in microbiology. v. 47, Immunological methods in microbiology / edited by Charles S. Pavia, Volker Gurtler. -- London : Elsevier, c2020. – (58.605 /N856 /v.47) |
Contents
Contributors
Preface
CHAPTER 1 Introduction to immunological
techniques in the clinical laboratory
1. Introduction
2. Basics of the immune response
3. Hypersensitivities
4. Survey of current immunological
techniques
5. Conclusion
References
CHAPTER 2 Combining antigen detection and
serology for the diagnosis of selected infectious diseases
1. Introduction and historical background
2. Overview of immunoassay development
3. Streptococcus pyogenes (Group A Strept)
4. Streptococcus pneumoniae
5. Legionella pneumophila
6. Helicobacter pylori
7. Coccidioides immitis
8. Cryptococcus neoformans
9. Summary
Financial disclosures and conflicts of
interest
References
Further reading
CHAPTER 3 Immunologic detection of Lyme
disease and the related borrelioses
1. Introduction
2. Microbiologic features of the Borrelia
bacteria
3. Lyme disease
4. Relapsing fever borreliosis
5. Southern tick-associated rash illness
Acknowledgements
Financial disclosures and conflicts of
interest
References
Further reading
CHAPTER 4 Immunodetection of bacteria
causing brucellosis.
1. Introduction
2. The genus Brucella
3. Epidemiology of Brucella
4. Disease in humans
5. Disease in animals
6. Diagnostic dilemma in brucellosis
7. Immune response to Brucella
8. Antigens of Brucella employed in immunodiagnosis
9. Immunodetection methods for brucellosis
10. Recent developments in brucellosis
research
Conclusions and perspective
References
Further reading
CHAPTER 5 Immunological and molecular
techniques used for determination of serotypes in Pasteurellaceae
1. Introduction
2. Serotyping
3. Discussion and conclusion
Acknowledgements
References
Further reading
CHAPTER 6 Immunological tests for diarrhoea
caused by diarrhoeagenic Escherichia coli targeting their main virulence
factors
1. Definition
2. Polyclonal antibodies generation
3. Monoclonal antibodies generation
4. Recombinant antibody generation .
5. Immunological assays
6. Summary
References
Further reading
CHAPTER 7 Development and improvement of a
colony blot immunoassay for the detection of thermotolerant Campylobacter
species
1. Introduction
2. Development and improvement of a CBI for
Campylobacter spp.: Methods
3. Results and discussion
4. Colony blot immunoassay protocol
Acknowledgements
References
CHAPTER 8 Serodiagnosis of Strongyloides
stercoralis infection
1. Strongyloides stercoralis
2. Strongyloidiasis
3. Laboratory diagnosis
4. Serological assays
5. Challenges and way forward
Acknowledgements
References
Further reading
CHAPTER 9 Immunological detection of Zika
virus: A summary in the context of general viral diagnostics
1. Introduction
2. Zika virus (ZIKV)
3. Understanding cross-reactivity
4. General diagnostics
5. Immunological diagnostics
6. Serological methods
7. Conclusion
References
CHAPTER 10 Detection of microorganisms using
recombinase polymerase amplification with lateral flow dipsticks
1. Introduction
2. Rational of RPA and LEA
3. Materials, equipment and reagents
4. Protocols for RPA-LEA
5. Solutions to potential problems
6. Conclusion and outlook of RPA
Acknowledgements
References
Further reading
CHAPTER 11 Detection of microorganisms with
lateral flow test strips
1. Introduction
2. Comparison of bacterial detection methods
3. Principles of LFIA
4, Recognition elements for stability
5. Detector for quantitative, uniform
analysis
6. Sensitivity improvement
7. Multi-step analysis
8. Multiplex analysis
9. Cross-reactive Abs and specificity
10. Label-free or immobilised-free format
11. Application of LFIA
12. Future prospects
13. Conclusions
Acknowledgement
References