Proteomic and Metabolomic Approaches to Biomarker Discovery / edited by Haleem J. Issaq, Timothy D. Veenstra. -- 2nd ed. -- Amsterdam : Academic Press, 2020. – (58.17421 /P967ac /2nd ed.)

Contents

Contributors  xi

Preface to the second edition  xv

1. Biomarker discovery: Study design and execution

2. Proteomic and mass spectrometry technologies for biomarker discovery

3. Tissue sample preparation for proteomic analysis

4. Sample preparation in global metabolomics of biological fluids and tissues

5. Serum and plasma collection: Preanalytical variables and standard operating procedures in biomarker research

6. Sample depletion, fractionation, and enrichment for biomarker discovery

7. Current NMR strategies for biomarker discovery

8. Gas chromatography/mass spectrometry- based metabonomics

9. Liquid chromatographic methods combined with mass spectrometry in metabolomics

10. Capillary electrophoresis-mass spectrometry for proteomic and metabolic analysis

11. Associating 2-DE and CPLLs for low-abundance protein discovery: A winning strategy

12. Two-dimensional difference in gel electrophoresis for biomarker discovery

13. Affinity-targeting schemes for protein biomarkers

14. Protein and metabolite identification

15. Quantitative proteomics in development of disease protein biomarkers

16. Mass spectrometry and NMR spectroscopy based quantitative metabolomics

17. Top-down mass spectrometry for protein molecular diagnostics, structure analysis, and biomarker discovery

18. Using data-independent mass spectrometry to extend detectable dynamic range without prior fractionation

19. Imaging mass spectrometry of intact biomolecules in tissue sections

20. Mass spectrometry-based approach for protein biomarker verification

21. Mass spectrometry metabolomic data handling for biomarker discovery

22. Analytical methods and biomarker validation

23. Multivariate analysis for metabolomics and proteomics data

24. Cell surface protein enrichment for biomarker and drug target discovery using mass spectrometry-based proteomics

25. Advances in lipidomics for cancer biomarker discovery

26. Mass spectrometry for the identification of protein biomarkers in urinary extracellular vesicles

27. Designing clinical studies for biomarker discovery: The Design criteria