Expression, purification, and structural biology of membrane proteins / edited by Camilo Perez and Timm Maier. -- New York : Humana Press, 2020.—(58.17/M592/v.2127)

Contents

Preface

Contributors

1  Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae

2  Membrane Protein Production in Escherichia coli

3  Membrane Protein Production in Lactococcus lactis for Structural Studies

4  Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae

5  Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System

6  Membrane Extracts from Plant Tissues

7  Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter

8  GPCR Solubilization and Quality Control

9  Affinity Purification of Membrane Proteins

10  Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage

11  Biotinylation of Membrane Proteins for Binder Selections

12  Production and Application of Nanobodies for Membrane Protein Structural Biology

13  Identifying Conformation-Selective Heavy-Chain-Only Antibodies Against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay

14  Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses

15  Reconstitution of Proteoliposomes for Phospholipid Scrambling and Nonselective Channel Assays

16  Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent

17  Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs

18  Fast Small-Scale Membrane Protein Purification and Grid Preparation for Single-Particle Electron Microscopy

19  Stabilization and Crystallization of a Membrane Protein Involved in Lipid Transport

20  In Meso In Situ Serial X-Ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source

21  Membrane Protein Preparation for Serial Crystallography Using High-Viscosity Injectors: Rhodopsin as an Example

22  Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins

23  Mechanical Unfolding and Refolding of Single Membrane Proteins by Atomic Force Microscopy

24  Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins

25  Exploring Lipid and Membrane Protein Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy

Index