Peptide nucleic acids : methods and protocols / edited by Peter E. Nielsen. -- 3rd ed. -- New York : Humana Press, 2020. – (58.17/M592/v.2105)

Contents

Preface

Contributors

1  Fmoc-Based Assembly of PNA Oligomers: Manual and Microwave-Assisted Automated Synthesis

2  A Robust Method for Preparing Optically Pure MiniPEG-Containing Gamma PNA Monomers

3  Synthesis of Pyrrolidinyl PNA and Its Site-Specific Labeling' at Internal Positions by Click Chemistry

4  Synthesis of PNA-Peptide Conjugates as Functional SNARE Protein Mimetics

5  Lipid-Modified Peptide Nucleic Acids: Synthesis and Application to Programmable Liposome Fusion

6  Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimide-Thioether Linkage

7  PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries

8  Transcription Inhibition by PNA-Induced R-Loops

9  Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA

10  In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA)

11  Reactive Quantum Dot-Based FRET Systems for Target-Catalyzed Detection of RNA

12  Peptide Nucleic Acids for MicroRNA Targeting

13  Detection of Microorganisms by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid

14  PNA Antisense Targeting in Bacteria: Determination of Antibacterial Activity (MIC) of PNA-Peptide Conjugates

15  In Vivo Administration of Splice Switching PNAs Using the mdx Mouse as a Model System

16  Near-Infrared In Vivo Whole-Body Fluorescence Imaging of PNA

17  Poly(Lactic-co-Glycolic Acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo

18  Preparation of Conjugates for Affibody-Based PNA-Mediated Pretargeting

Index