Expressed protein ligation : methods and protocols / edited by Miquel Vila-Perello. -- New York : Humana Press, c2020. – (58.17/M592/v.2133)

Contents

1  Methods and Applications of Expressed Protein Ligation

2  Protein Splicing: From the Foundations to the Development of Biotechnological Applications

3  Identification, Characterization, and Optimization of Split Inteins

4  Methods to Study the Structure and Catalytic Activity of cis-Splicing Inteins

5  Expressed Protein Ligation: General Experimental Protocols

6  Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins

7  Native Chemical Ligation via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid-Phase Peptide Synthesis

8  Fused Split Inteins: Tools for Introducing Multiple Protein Modifications

9  Streamlined Expressed Protein Ligation: Site-Specific Antibody-Drug Conjugate

10  In Vivo Histone Labeling Using Ultrafast trans-Splicing Inteins

11  Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation

12  Site-Specific Phosphorylation of PDZ Domains

13  Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications

14  Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2 -Aminooxyethanethiol

15  Investigating the Effects of O-GlcNAc Modifications in Parkinson's Disease Using Semisynthetic α-Synuclein

16  Recombinant Expression of Cyclotides Using Expressed Protein Ligation

17  Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines.

Index