Metabolic signaling : methods and protocols / edited by Sarah-Maria Fendt, Sophia Y. Lunt. -- New York : Humana Press, c2019. – (58.17/M592/v.1862)

Contents

Preface

Contributors

1  A Protocol to Compare Methods for Untargeted Metabolomics

2  High-Throughput Measure of Bioactive Lipids Using Non-targeted Mass Spectrometry

3  Measuring the Nutrient Metabolism of Adherent Cells in Culture

4  13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells

5  Measuring In Vivo Tissue Metabolism Using 13C Glucose Infusions in Mice

6  Measuring Human Lipid Metabolism Using Deuterium Labeling: In Vivo and In Vitro Protocols

7  Measuring Rates of ATP Synthesis

8  Direct Estimation of Metabolic Flux by Heavy Isotope Labeling Simultaneous with Pathway Inhibition: Metabolic Flux Inhibition Assay

9  Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers

10  Determining Compartment-Specific Metabolic Fluxes

11  Determining the Impact of Metabolic Nutrients on Autophagy

12  Measuring the Activation of Cell Death Pathways upon Inhibition of Metabolism

13  Determining Macrophage Polarization upon Metabolic Perturbation

14  Assessing the Impact of the Nutrient Microenvironment on the Metabolism of Effector CD8+ T Cells

15  Development of Patient-Derived Tumor Xenograft Models

16  Imaging Glioma Progression by Intravital Microscopy

17  Lipectomizing Mice for Applications in Metabolism

18  Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants.

19  Determining the Intracellular Organization of Organelles

20  The Fundamentals of Constructing and Interpreting Heat Maps

Index