Functional proteomics : methods and protocols / edited by Xing Wang, Matthew Kuruc. -- New York : Humana Press, c2019. – (58.17/M592/v.1871)

Contents

Preface

Contributors

1  Making the Case for Functional Proteomics

2  Methods to Monitor the Functional Subproteomes of SERPIN Protease Inhibitors

3  Two-Dimensional 16-BAC/SDS Polyacrylamide Gel Electrophoresis of Mitochondrial Membrane Proteins

4  Systematic Glycolytic Enzyme Activity Analysis from Human Serum with PEP Technology

5  A Protein Decomplexation Strategy in Snake Venom Proteomics

6  Fractionation Techniques to Increase Plant Proteome Coverage: Combining Separation in Parallel at the Protein and the Peptide Level

7  A Systematic Analysis Workflow for High-Density Customized Protein Microarrays in Biomarker Screening

8  Metaproteomics Study of the Gut Microbiome

9  Double One-Dimensional Electrophoresis (DI-DE) Adapted for Immunoproteomics

10  BioID: A Proximity-Dependent Labeling Approach in Proteomics Study.

11  Functional Application of Snake Venom Proteomics in In Vivo Antivenom Assessment

12  Proteomic Detection of Carbohydrate-Active Enzymes (CAZymes) in Microbial Secretomes

13  An Overview of Mass Spectrometry-Based Methods for Functional Proteomics

14  Functional Proteomic Analysis to Characterize Signaling Crosstalk

15  Identification of Unexpected Protein Modifications by Mass Spectrometry-Based Proteomics

16  Label-Free LC-MS/MS Strategy for Comprehensive Proteomic Profiling of Human Islets Collected Using Laser Capture Microdissection from Frozen Pancreata

17  Targeted Proteomics

18  Metabolomic Investigation of Staphylococcus aureus Antibiotic Susceptibility by Liquid Chromatography Coupled to High-Resolution Mass Spectrometry

19  Nuts and Bolts of Protein Quantification by Online Trypsin Digestion Coupled LC-MS/MS Analysis

20  Proteases: Pivot Points in Functional Proteomics

21  The Use of Combinatorial Hexapeptide Ligand Library (CPLL) in Allergomics

22  Efficient Extraction and Digestion of Gluten Proteins

23  Glycosylation Profiling of Tumor Marker in Plasma Using Bead-Based Immunoassay

24  Protein-Specific Analysis of Invertebrate Glycoproteins

25  The Use of Proteomics Studies in Identifying Moonlighting Proteins

26  Two-Dimensional Biochemical Purification for Global Proteomic Analysis of Macromolecular Protein Complexes

27  A Data Analysis Protocol for Quantitative Data-Independent Acquisition Proteomics

Index