Autophagy in differentiation and tissue maintenance : methods and protocols / edited by Kursad Turksen. -- New York : Humana Press, c2019. – (58.17/M592/v.1854)

Contents

Contributors

Visualization and Measurement of Multiple Components of the Autophagy Flux

Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells

Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells

Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement

Autophagy in Adipocyte Differentiation

Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells

Assessing Autophagy in the Leydig Cells

Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-small Cell Lung Cancer Cells

Autophagy in Zebrafish Extraocular Muscle Regeneration

The Detection Techniques for Autophagy-Associated Cell Death-Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry

Cloning of Autophagy-Related MicroRNAs

Assays to Monitor Aggrephagy in Drosophila Brain

Methods for Monitoring Autophagy in Silkworm Organs

Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, and ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia

Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening

Porcine Cell-Free System to Study Mammalian Sperm Mitophagy

Monitoring and Measuring Mammalian Autophagy

Index