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Analytical techniques in biochemistry / by Mahin Basha. -- New York : Humana Press, c2020. – (58.173/B299) |
Contents
1 Sedimentation
1 Gravity Sedimentation
Reference
2 Filtration
1 Types of Filtration
2 Microfiltration
3 Ultrafiltration
Reference
3 Centrifugation
1 Two Basic Modes of Centrifugation
2 Instrument Design
References
4 Colorimetry and Spectrophotometer (Spectrophotometry)
1 Introduction
2 Colorimetry
3 Beer's and Lambert's Law
4 Deviation in Beer's and Lambert's Law
5 Instrumentation of Colorimeter
6 Spectrophotometry
7 Filter and Monochromators
8 Applications
References
5 Chromatography
1 Introduction
2 Types of Chromatography
3 Partition Chromatography
4 Uses for Chromatography
5 Column Chromatography
6 Parameters of HPLC
7 Instrumentation
8 Chromatogram of Petrol
9 Gel Filtration Chromatography
References
6 Electrophoresis
1 Supporting Media for Electrophoresis
2 Factors Affecting Electrophoretic Mobility
3 Structure Shows Types of Electrophoresis
4 Paper Electrophoresis
5 Detection and Quantitative Assay
6 SDS-Polyacrylamide Gel Electrophoresis
7 SDS-Polyacrylamide Gel Electrophoresis
8 Immunoelectrophoresis
References
7 Immuno-techniques
1 Introduction
2 Immunodiffusion
3 Immuno-fluorescence
4 Immunoassay: Enzyme-Linked Immunosorbent Assay (ELISA)
5 Radioimmunoassay (RIA)
6 Western Blotting or Immunoblotting
7 Southern Blotting
8 Northern Blot: RNA-DNA*(RNA*)
References
8 Radioactivity
1 Radiation Types
2 Measurement of Radioactivity
3 Types
4 Excitation of Solids and Liquids
5 Ability to Expose Photographic Films (Autoradiography)
6 Tracer Technique
7 Clinical and Biomedical Uses of Radioactive Substances
8 Health Effects of Radiations
Reference
9 Microscopy and Specimen Preparation
1 The Light Microscope
2 Preparation and Staining of Specimens
3 Electron Microscopy
4 Principles
5 Preparation of Specimens
6 Characteristics of SEM
7 TEM
8 Applications
References
Index
