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Brain development : methods and protocols / edited by Simon G. Sprecher. -- 2nd ed. -- Totowa, NJ, United States : Humana Press, 2020. – (58.17 /M592 /v.2047) |
Contents
Preface
Contributors
PART I
INVERTEBRATE MODELS
1
Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor
Adult Neurogenesis in Hydra
2
Reverse Genetic Approaches to Investigate the Neurobiology of the
Cnidarian Sea Anemone Nematostella vectensis
3
Generating Transgenic Reporter Lines for Studying Nervous System
Development in the Cnidarian Nematostella vectensis
4 Immunostaining and In Situ Hybridization of
the Developing Acoel Nervous System
5
Immunostaining of the Embryonic and Larval Drosophila Brain
6 Nonfluorescent
RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple
Gene Expression Patterns in the Embryonic Brain of Drosophila
7 Analysis
of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI
Labeling
8
Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update..
9
Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain...
10 CRISPR/Cas9 Genome Editing to Study Nervous
System Development in Drosophila
11
The Red Flour Beetle as Model for Comparative Neural Development: Genome
Editing to Mark Neural Cells in Tribolium Brain Development
12 A
Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry
for the Study of Embryonic Brain Development in Tribolium castaneum
13 Immunohistochemistry
and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult
Brains of Tribolium
14 X-Ray Microscopy of the Larval Crustacean
Brain
15
Immunolocalization of Neurotransmitters and Neuromodulators in the
Developing Crayfish Brain
16 Immunostainings
in Nervous System Development of the Nematode C. elegans
17 Methods in Brain Development of Molluscs
18 A
Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages
Following In Situ Hybridization
19 Spawning
Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma
lanceolatum
PART II
VERTEBRATE MODELS
20 In
Situ Hybridization and Immunostaining of Xenopus Brain
21
Morpholino Studies in Xenopus Brain Development
22 Sensitive
Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal
Amplification and Its Combination with Immunofluorescent Protein Visualization
in Zebrafish
23
Live Morphometric Classification of Sensory Neurons in Larval Zebrafish
24 Immunohistochemistry
and In Situ Hybridization in the Developing Chicken Brain
25
Gene-Silencing in Chicken Brain Development
26 Transplantation
of Neural Tissue: Quail-Chick Chimeras
27 Immunohistochemistry
and RNA In Situ Hybridization in Mouse Brain Development
28 The
Cre/Lox System to Assess the Development of the Mouse Brain
29 In
Utero Electroporation to Study Mouse Brain Development
Index
