Biomolecular and bioanalytical techniques : theory, methodology and applications / edited by Vasudevan Ramesh. -- Hoboken, NJ : Wiley, 2019. – (58.178/B615t)

Contents

List of Contributors  xix

Preface  xxiii

1      Principles of Health and Safety and Good Laboratory Practice  1

2      Applications of Chemoinformatics in Drug Discovery  17

3      Bioinformatics and Its Applications in Genomics  37

4      Gene Cloning for the Analysis of Gene Expression  59

5      Proteomic Techniques and Their Applications  81

6      Overproduction, Separation and Purification of Affinity-Tagged Proteins from Escherichia coli  101

7      Chromatography: Separation Techniques in Biology  123

8      Synthetic Methodology in Chemical Biology  153

9      Reaction Chemical Kinetics in Biology  179

10     Mass Spectrometry and Its Applications  2,19

11     Applications and Complementarity of Analytical Ultracentrifugation and Light-Scattering Techniques  255

12     Application of Isothermal Titration Calorimetry (ITC) to Biomolecular Interactions  279

13     An Introduction to Infra-red and Raman Spectroscopies for Pharmaceutical and Biomedical Studies  307

14     Fluorescence Spectroscopy and Its Applications in Analysing Biomolecular Processes  333

15     Circular Dichroism and Related Spectroscopic Techniques  365

16     Principles and Practice in Macromolecular X-Ray Crystallography  385

17     Biomolecular NMR Spectroscopy and Structure Determination of DNA  421

18     Cryo-TEM and Biological Structure Determination  471

19     Computer Modelling and Molecular Dynamics Simulation of Biomolecules  501

 Index  537