Cell biology : new insights / [edited by] Prerna Pandey. -- New York : Delve Publishiing, c2017 .—(58.15 /C393n)

Contents

List of Contributors

Preface

Chapter 1  Plasma Proteome Profiling to Assess Human Health and Disease  1

1.  Introduction  1

2.  Results  2

3.  Discussion    5

Acknowledgments    6

References  6

Chapter 2  Biological Membranes

Abstract

1.  Structure And Organization Of Membranes

2.  Membrane Proteins Control What Enters And Leaves The Cell

3.  Solving The Structure Of Membrane Proteins

4.  Interactions Between Lipids And Proteins In Biological Membranes

5.  Internal Membranes In Eukaryotic Ceils Form Organelles

6.  Sending Messages Across Membranes

7.  Membranes In Health And Disease

References

Chapter 3  Oriented Cell Division: New Roles in Guiding Skin Wound Repair and Regeneration

Abstract

1.  Introduction

2.  Oriented Cell Division in the Development Of Organisms

3.  Mechanisms of Spindle Orientation in Ce[l Division

4.  Oriented Cell Division in Skin

5.  Perspectives

References

Chapter 4  Motility-Driven Glass and Jamming Transitions in Biological Tissues  37

Abstract  37

1.  Introduction    37

2.  Spy Model  38

3.  Characterizing Glassy Behavior  39

4.  A Continuum Model for Glass Transitions in Tissues  42

5.  Discussion and Conclusions  42

References  44

Chapter 5  Dendritic Cells in Autoimmune Diseases

Abstract

1.  Subsets of Dendritic Cells

2.  Maturation and Activation of Dendritic Cells

3.  Induction of Adaptive Immune Responses by Dendritic Cells

4.  Dendritic Cells in Immune Tolerance: Generation of Regulatory T Ceils

5.  Dendritic Cells: Inciting and Sustaining Autoimmunity

6. Dendritic Cells in Autoimmune Diseases: Few Specific Examples  51

Conclusion  52

Acknowledgements  52

References  52

Chapter 6  The Longevity of the Humoral Immune Response: Survival of Long-Lived Plasma Cells

Abstract  55

1.  Introduction  55

2.  Development of Long-Lived Plasma Ceils  57

3.  Extrinsic Factors for Survival Secreted by the Bone Marrow Microenvironment  58

4.  The Bone Marrow Microenvironment as a Survival Niche for LPCS  59

Conclusion

Acknowledgements

References

Chapter 7  Review of Quantitative Phase-Digital Holographic Microscopy: Promising Novel Imaging Technique to Resolve Neuronal Network Activity and Identify Cellular Biomarkers of Psychiatric Disorders

Abstract  65

1.  Introduction  65

2.  Principles of Quantitative Phase Measurements Based on Digital Holographic Microscopy  66

3.  Cell Imaging With Quantitative Phase Microscopy  69

4.  Resolving Neuronal Network Activity and Visualizing Spine Dynamics  70

5.  Quantitative Phase Digital Holographic Microscopy: Tool to Screen and Identify new Cell Biomarkers of Psychiatric Disorders  '75

6.  Perspectives  76

Acknowledgments  77

References  77

Chapter 8  Transcriptomics Resources of Human Tissues and Organs Abstract  85

1.  Introduction  85

2.  Analyses of the Human Transcriptome by Different Methods  87

3.  Classification of All Human Protein-Coding Genes Based on Tissue Profiling  88

4.  Tissue-Enriched Genes Vs. Ubiquitously Expressed Genes  89

5.  The Variation in Protein Profiles Between Individuals  90

6.  Building Genome-Scale Metabolic Models for Human Tissues    91

7.  Applying Context-Dependent Gems for Analyzing Human Diseases    93

Concluding Remarks  93

Acknowledgements

References

Chapter 9  Biology, Detection, and Clinical Implications of Circulating Tumor Cells

Abstract

1.  Biology of Circulating Tumor Cells  99

2.  Enrichment and Detection of CTCS  102

3.  Clinical Implications of Circulating Tumor Cells  104

4.  Conclusions and Outlook  105

References

Chapter 10  The Kynurenine Pathway in Stem Cell Biology

Abstract

1.  Introduction

2.  Evidence of KP Involvement in Stem Cell Biology

Conclusion

Author Contributions.

Disclosures and Ethics

References

Chapter 11  Reprogrammable Crispr/Cas9-Based System for Inducing Site-Specific DNA Methylation

Abstract

1.  Introduction

2.  Results

3.  Discussion

4.  Materials and Methods

Acknowledgements

Footnotes

References

Chapter 12  Technical Advance: Transcription Factor, Promoter, and Enhancer Utilization in Human Myeloid Cells

1.  Introduction

2.  Materials and Methods

3.  Results

4.  Discussion

5.  Authorship

Acknowledgments

References

Chapter 13  Application of Chromogenic in Situ Hybridization (CISH) for Human Papillomavirus (HPV) Genotyping

Abstract

1.  Introduction

2.  Methods

3.  Results

4.  Discussion

5.  Funding

References

Chapter 14  Molecular Classification of Cancer with The 92-Gene Assay in Cytology and Limited Tissue Samples

Abstract

1.  Introduction

2.  Results

3.  Discussion

4.  Materials and Methods

Acknowledgments

Conflicts of Interest

References

Chapter 15  Changing Perspective on Oncometabolites : From Metabolic Signature of Cancer to Tumorigenic And Immunosuppressive Agents

Abstract

1.  Introduction

2.  Classic Oncogenic Signals Divert Metabolism from Oxidative Phosphorylation to Glycolysis  161

3.  When Mitochondria are Upstream of Cancer Development  161

4.  Cancer-Related Inflammation is Driven by Oncometabolites  162

5.  A Closer Look at Tumour-Derived Lactic Acid: How Turnout-Associated Macrophages are Functionally Polarized  163

6.  Metabolic Competition Controls Anti-Tumoral Function of T Lymphocytes  164

Conclusions  165

Acknowledgments

Funding

References

Chapter 16  Insights In Cell Science -Towards a Quantitative Approach of Fluorescence Microscopy that Unravels Cellular Function

1.   Mini Review  173

Acknowledgements  175

References  175

Chapter 17  Mesenchymal Stern Cell and Osteoarthritis: A Literature Review

Abstract  177

1.  Introduction  177

2.  Literature Review  178

Conclusion  178

Acknowledgement  179

References    179

Chapter 18  Rational Design of Organelle Compartments in Cells

1.  Motivation and Objectives

2.  Methods

3.  Results and Discussion  182

References  182

Chapter 19  Organ-On-A-Chip: New Platform for Biological Analysis

Abstract  185

1.  Introduction  185

2.  Heart-On-a-Chip  185

3.  Vessel-On-a-Chip  186

4.  Liver-On-a-Chip    187

5.  Neuron-On-a-Chip  187

6.  Kidney-On-a-Chip    188

7.  Multi-Organs-On-a-Chip  188

8.  Conclusion and Perspectives  189

9.  Funding  189

10. Competing Interests    189

References  189

Chapter 20  Advances in Improving Mammalian Cells Metabolism for Recombinant Protein Production

Abstract

1.  Introduction

2.  Characteristics of the Energetic Metabolism of Mammalian Cell Lines

3.  Improving Metabolism: Alternative Metabolisable Substrates and Cell Engineering

Concluding Remarks

References

Index