Cell Engineering / Li Zhiyong. -- 2nd ed. -- Beijing : Science Press; Oxford : Alpha Science International Ltd, c2014. – (58.22 /L693 /2nd ed.)

Contents

Preface

Part 1  Fundamentals of Cell Engineering

Chapter 1  Introduction to Cell Engineering  3

     1.1  Bioengineering  3

     1.2  Cell Engineering  7

Chapter 2  Cell Engineering Basics  14

     2.1  Composition of Cells  14

     2.2  Cell Cycle & Division  ~  20

     2.3  Cell Apoptosis & Cytoclasis  23

     2.4  Differentiation&Dedifferentiation  24

     2.5  Tissue & Organ  26

     2.6  Reproduction &Development  26

Part 2  Plant Cell Engineering

Chapter 3  Plant Tissue & Organ Culture  35

     3.1  Plant Tissue Culture  35

     3.2  Plant Embryo Culture  48

     3.3  Hairy Root Culture  49

Chapter 4  Artificial Seeds & Virus-Free of Plants  55

     4.1  Artificial Seeds  55

     4.2  Virus Elimination of Plants  57

Chapter 5  Plant Cell Culture & Preparation of Secondary Metabolites  62

     5.1  Plant Cell Culture and Its Characteristics  62

     5.2  Plant Cell Culture Techniques  63

     5.3  Production of Secondary Metabolites by Plant Cell Culture  73

Chapter 6  Protoplast Culture & Mutagenesis  80

     6.1  Protoplast Culture  80

     6.2  Protoplast Mutagenesis  83

Chapter 7  Cell Fusion & Somatic Cell Hybridization  85

     7.1  Cell Fusion  85

     7.2  Somatic Hybridization  90

Chapter 8  Polyploid and Haploid Plants  94

     8.1  Chromosome Engineering  94

     8.2  Polyploid Plants  94

     8.3  Haploids and Homozygous Diploid Plants  98

Chapter 9  In Vitro Fertilization  104

     9.1  Plant in Vitro Fertilization  104

     9.2  In Vitro Pollination  105

     9.3  In Vitro Fertilization  105

Chapter 10  Transgenic Techniques  108

     10.1  Transgenic Plants  108

     10.2  Genetically Modified Plants  113

     10.3  Biopharmaceutical Transgenic Plants  114

     10.4  Safety of Transgenic Plants  115

Part 3  Animal Cell Engineering

Chapter 11  Animal Cell Culture & Preparation and Expression of Medicinal Proteins

     11.1  Animal Cell Culture  119

     11.2  Characteristics of Animal Cell Culture  120

     11.3  Culture Tools and Conditions  123

     11.4  Growth of in Vitro Cultured Cells  128

     11.5  Cell Line/Strain and Preservation  132

     11.6  Small-Scale Animal Cell Culture Methods  135

     11.7  Large-Scale Animal Cell Culture  137

     11.8  Animal Cell Culture Bioreactors  141

     11.9  Factors of Large-Scale Animal Cell Culture  143

     11.10  Preparation of Pharmaceutical Proteins with Animal Cells  ... 146

Chapter 12  Hybridoma Technology and Monoclonal Antibody  160

     12.1  Hybridoma Technology  160

     12.2  Monoclonal Antibody  161

     12.3  Humanized Monoclonal Antibody  167

Chapter 13  In Vitro Fertilization & Animals Cloned by Nuclear Transfer   ... 170

     13.1  Embryo Technique  170

     13.2  IVF Animals  170

     13.3  Test-Tube Baby  -  176

     13.4  Animals Cloned by Nuclear Transfer  179

     13.5  Cryopreservation Technique  185

     13.6  Mammalian Sex Control  190

Chapter 14  Polyploid and Transgenic Animals  194

     14.1  Polyploid Animals  194

     14.2  Transgenic Animals  196

Chapter 15  Stem Cells  202

     15.1  Characteristics of Stem Ceils  202

     15.2  Embryonic Stem Cells  203

     15.3  Adult Stem Cells  209

Chapter 16  Tissue Engineering  213

16.1  Tissue Engineering and Its Three Elements  213

16.2  Technical Routes of Tissue Engineering  217

16.3  Tissue Engineering Bioreactors  218

16.4  Tissue Engineering Products  219

Index  221