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Cell Engineering / Li Zhiyong. -- 2nd ed. -- Beijing : Science Press; Oxford : Alpha Science International Ltd, c2014. – (58.22 /L693 /2nd ed.) |
Contents
Preface
Part 1 Fundamentals of Cell Engineering
Chapter 1 Introduction to Cell Engineering 3
1.1 Bioengineering 3
1.2 Cell Engineering 7
Chapter 2 Cell Engineering Basics 14
2.1 Composition of Cells 14
2.2 Cell Cycle & Division ~ 20
2.3 Cell Apoptosis & Cytoclasis 23
2.4 Differentiation&Dedifferentiation 24
2.5 Tissue & Organ 26
2.6 Reproduction &Development 26
Part 2 Plant Cell Engineering
Chapter 3 Plant Tissue & Organ Culture 35
3.1 Plant Tissue Culture 35
3.2 Plant Embryo Culture 48
3.3 Hairy Root Culture 49
Chapter 4 Artificial Seeds & Virus-Free of Plants 55
4.1 Artificial Seeds 55
4.2 Virus Elimination of Plants 57
Chapter 5 Plant Cell Culture & Preparation of Secondary Metabolites 62
5.1 Plant Cell Culture and Its Characteristics 62
5.2 Plant Cell Culture Techniques 63
5.3 Production of Secondary Metabolites by Plant Cell Culture 73
Chapter 6 Protoplast Culture & Mutagenesis 80
6.1 Protoplast Culture 80
6.2 Protoplast Mutagenesis 83
Chapter 7 Cell Fusion & Somatic Cell Hybridization 85
7.1 Cell Fusion 85
7.2 Somatic Hybridization 90
Chapter 8 Polyploid and Haploid Plants 94
8.1 Chromosome Engineering 94
8.2 Polyploid Plants 94
8.3 Haploids and Homozygous Diploid Plants 98
Chapter 9 In Vitro Fertilization 104
9.1 Plant in Vitro Fertilization 104
9.2 In Vitro Pollination 105
9.3 In Vitro Fertilization 105
Chapter 10 Transgenic Techniques 108
10.1 Transgenic Plants 108
10.2 Genetically Modified Plants 113
10.3 Biopharmaceutical Transgenic Plants 114
10.4 Safety of Transgenic Plants 115
Part 3 Animal Cell Engineering
Chapter 11 Animal Cell Culture & Preparation and Expression of Medicinal Proteins
11.1 Animal Cell Culture 119
11.2 Characteristics of Animal Cell Culture 120
11.3 Culture Tools and Conditions 123
11.4 Growth of in Vitro Cultured Cells 128
11.5 Cell Line/Strain and Preservation 132
11.6 Small-Scale Animal Cell Culture Methods 135
11.7 Large-Scale Animal Cell Culture 137
11.8 Animal Cell Culture Bioreactors 141
11.9 Factors of Large-Scale Animal Cell Culture 143
11.10 Preparation of Pharmaceutical Proteins with Animal Cells ... 146
Chapter 12 Hybridoma Technology and Monoclonal Antibody 160
12.1 Hybridoma Technology 160
12.2 Monoclonal Antibody 161
12.3 Humanized Monoclonal Antibody 167
Chapter 13 In Vitro Fertilization & Animals Cloned by Nuclear Transfer ... 170
13.1 Embryo Technique 170
13.2 IVF Animals 170
13.3 Test-Tube Baby - 176
13.4 Animals Cloned by Nuclear Transfer 179
13.5 Cryopreservation Technique 185
13.6 Mammalian Sex Control 190
Chapter 14 Polyploid and Transgenic Animals 194
14.1 Polyploid Animals 194
14.2 Transgenic Animals 196
Chapter 15 Stem Cells 202
15.1 Characteristics of Stem Ceils 202
15.2 Embryonic Stem Cells 203
15.3 Adult Stem Cells 209
Chapter 16 Tissue Engineering 213
16.1 Tissue Engineering and Its Three Elements 213
16.2 Technical Routes of Tissue Engineering 217
16.3 Tissue Engineering Bioreactors 218
16.4 Tissue Engineering Products 219
Index 221