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Immunocytochemistry and related techniques / edited by Adalberto Merighi and Laura Lossi. -- New York : Humana Press, c2015. – (63.17/I33r) |
Contents
Series Preface
Preface
Contributors
1 The
Evolution of Immunocytochemistry in the Dissection of Neural Complexity
PART I
IMMUNOCYTOCHEMISTRY IN NON-MAMMALIAN BRAIN
2
Immunofluorescence and Genetic Fluorescent Labeling Techniques in the
Drosophila Nervous System 39
3
Immunohistochemical Analysis of Neuronal Networks in the Nervous System
of Octopus vulgar# 63
4 Genetic
Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain 81
PART II
DETECTION OF CELL PROLIFERATION AND NEUROGENES1S
5
Immunocytochemical Phenotype of Differentiating Neurons 95
6
Immunocytochemical Identification of Mammalian Differentiating Neurons
in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus 109
7 Identification
of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogs:
Benefits and Limitations 123
8 Flow
Cytometry-Based Quantification of Neurogenesis in the Central Nervous
System 141
PART III
IDENTIFICATION OF SPECIFIC CELL TYPES AND INTRACELLULAR PATHWAYS
9 In Vivo
and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis and
Autophagy
10 Immunocytochemical
Detection of Intraneuronal Aβ Peptides in Mouse Models of
Alzheimer's Disease 179
11 Purification
and Characterization of Mouse Olfactory Ensheathing Cells and Their Potential
Use for Therapy in Amyotrophic Lateral Sclerosis (ALS) 195
12 Immunocytochemistry
of Microglial Cells 209
13 Immunocytochemical
Assessment of Blood-Brain Barrier Structure, Function, and Damage 225
PART IV
QUANTIFICATION OF IMMUNOCYTOCHEMISTRY AND COMBINED TECHNIQUES
14 Quantification of Immunocytochemical
Colocalization in Neurons 257
15
Immunogold Electron Microscopic Quantification of Small Molecular
Compounds and Proteins at Synapses and Other Neural Profiles 281
16 Combined
Immunocytochemistry and Tracing of Neural Connections 299
17 Neuronal
Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing and
Electrophysiology 313
18 Transfection
Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic
Slices 329
19 Combined
Immunochemistry and Live Imaging of Fluorescent Protein Expressing Neurons in
Mouse Brain
PART V NOVEL
APPROACHES
20 Array
Tomography: A Novel High-Resolution Immunofluorescence Technique
21 Single Molecule Localization Microscopy to Study
Neuronal Microtubule Organization
22 Studying
Membrane Dynamics of Proteins Using Quantum Dots 409
23 Optical
Protein Patterning 423
Index 437
