PCR technology : current innovations / edited by Tania Nolan, Stephen A. Bustin. -- 3rd ed. -- Boca Raton, Fla. : CRC Press, c2013. – (58.174252/P783r/3rd ed.)

Contents

    CONTENTS
    
    Template Preparation Pre-PCR Processing Strategies;
     Johannes Hedman， Maria Lovenklev， Petra Wolffs， Charlotta Lofstrom， Rickard Knutsson， and Peter Radstrom
    Use of a Liposomal Internal Control Vehicle for Whole-Process Quality Assurance of Nucleic Acid Amplification;
     Einar S. Berg and Tania Nolan
    Reaction Components Oligonucleotide Synthesis and Purification;
     Yannick Fillon， Theresa Creasey， Qin Xiang， and Carlos Martinez
    Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction;
     Ernest J. Mueller
    Real-Time Fluorescent PCR by Labeled Primer with a Single Fluorescent Molecule;
     Hong Yan and Liu Xiao Kun
    Using Scorpion Primers for Genotyping;
     David Whitcombe
    LNA: Adding New Functionality to PCR;
     Soren M. Echwald， Ditte Andreasen， and Peter Mouritzen
    Modified dNTPs: A Toolbox for Use in PCR;
     Natasha Paul， Olga Adelfinskaya， Elena Hidalgo Ashrafi， Tony Le， and Sabrina Shore
    Instruments Thermocycler Calibration and Analytical Assay Validation;
     Mary Span， Marc Verblakt， and Tom Hendrikx
    Ultra-High-Speed PCR Instrument Development;
     Nick Burroughs and Emmanouil Karteris
    Dropletization of Bioreactions: How Single-Cell Quantitative Analyses May Improve Gene Measurement and Cancer Diagnosis;
     Ehsan Karimiani， Amelia Markey， and Philip Day
    Design， Optimization， QC， and Standardization Assay Design for Real-Time qPCR;
     Gregory L. Shipley
    Primer Design for Large-Scale Multiplex PCR and Arrayed Primer Extension;
     Maido Remm， Kaarel Krjutskov， and Andres Metspalu
    Development and Use of qPCR Assays for Detection and Study of Neglected Tropical and Emerging Infectious Diseases;
     Ashley R. Heath， Norha Deluge， Maria Galli de Amorim， and Emmanuel Dias-Neto
    MIQE: Guidelines for Reliable Design and Transparent Reporting of Real-Time PCR Assays;
    Tania Nolan and Stephen A. Bustin
    Data Analysis Hypothesis-Driven Approaches to Multivariate Analysis of qPCR Data;
     Anders Bergkvist， Azam Sheikh Muhammad， and Peter Damaschke
    Experiment Design， Data Management， and Univariate Statistical Analysis of Gene-Expression Data Obtained by Real-Time Quantitative PCR;
    Ales Tichopad， Tzachi Bar， Malin Edling， Boel Svanberg， Tania Nolan， and Anders Bergkvist
    Biomarker Discovery via RT-qPCR and Bioinformatical Validation;
     Christiane Becker， Irmgard Riedmaier， and Michael W. Pfaffl
    Optimization of Quantitative Real-Time PCR for Studies in Cartilage Mechanobiology;
     Oto Akanji， Donald Salter， and Tina Chowdhury
    Applications Enriching DNA Sequences with Nucleotide Variation by Thymidine Glycosylases Combined with Suppression PCR;
     Xinghua Pan， Alexander E. Urban， and Sherman M. Weissman
    Chromosome Walking by Inverse PCR;
     Michael Gotesman， Selwyn A. Williams， Jorge A. Garces， and Ray H. Gavin
    Expression Profiling of MicroRNAs by Quantitative Real-Time PCR: The Good， the Bad， and the Ugly;
     Mirco Castoldi， Paul Collier， Tania Nolan， and Vladimir Benes
    Dye-Based High-Throughput qPCR in Microfluidic Platform BioMark(t);
     David Svec， Vendula Rusnakova， Vlasta Korenkova， and Mikael Kubista
    Surface Plasmon Resonance-Based Biosensor Technology for Real-Time Detection of PCR Products;
     Giordana Feriotto and Roberto Gambari
    Current Innovations in the Development and Application of the qPCR in a Resource-Limited Setting;
     Clare Watt and Jim Huggett
    A Review of Isothermal Nucleic Acid Amplification Technologies;
     Gavin J. Nixon and Claire A. Bushell
    Proximity Ligation Assay for Protein Quantification;
     David W. Ruff， Mark E. Shannon， Shiaw-Min Chen， Simon Fredriksson， and Ulf Landegren
    High-Resolution Melt Analysis;
     Einar S. Berg and Tania Nolan
    Index