Somatic stem cells : methods and protocols / edited by Shree Ram Singh. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.879)

Contents

    Contents
    
    Preface
    Contributors.
    PART I INTRODUCTION
    1 Current Thoughts on the Therapeutic Potential of Stem Cell
    PART II STEM CELL STUDY IN LOWER ORGANISM
    2 A Unique FACS Method to Isolate Stem Cells in Planarian
    3 Identification of Neural Stem Cells in the Drosophila Larval Brain
    4 Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut
    PART III STEM CELL STUDY IN MURINE AND HUMAN MODEL
    5 Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells
    6 Identification， Isolation， and Culture of Intestinal Epithelial Stem Cells from Murine Intestine
    7 Isolation and Characterization of Distal Lung Progenitor Cells
    8 Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells 123
    9 Convenient and Efficient Enrichment of the CD 133+ Liver Cells from Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells 135
    10 Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases.
    11 Functional Identification of Neural Stem Cell-Derived Oligodcndrocytes
    12 Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization
    13 A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures
    14 Isolation， Culture， and Osteogenic/Chondrogenic DiffErentiation of Bone Marrow-Derived Mesenchymal Stem Cells
    15 Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue
    16 Collection， Processing， and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine
    17 Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells
    18 Isolation and Characterization of Human Prostate Stem/Progenitor Cells
    19 Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts
    20 Isolation and DiffErentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes
    21 Isolation， Expansion， and Characterization of Human Islet-Derived Progenitor Cells
    22 Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli
    23 Endothelial Colony-Forming Progenitor Cell Isolation and Expansion
    24 Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes
    25 Human Salivary Gland Stem Cells: Isolation， Propagation， and Characterization
    26 Identification， Isolation， Characterization， and Banking of Human Dental Pulp Stem Cells 443
    27 Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin 465
    28 Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression 471
    29 Genetic Modification of Mesenchymal Stem Cells
    30 Methodology， Biology and Clinical Applications of Human Mesenchymal Stem Cells
    31 In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells
    32 Methods for Cancer Stem Cell Detection and Isolation
    33 Biocompatible Nanoparticle Labeling of Stem Cells and Their Distribution in Brain 531
    Index