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Nanoparticles in biology and medicine : methods and protocols / edited by Mikhail Soloviev. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.906) |
Contents
Contents
Contributors
PART I NOBLE METAL NANOPARTICLES: SYNTHESIS AND APPLICATIONS
1 Procedures for the Synthesis and Capping of Metal Nanoparticles
2 Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications
3 Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis
4 Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays
5 Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues
6 RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Color Multiplexing and Application to Cancer Diagnostics
7 Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins
PART II SEMICONDUCTOR NANOPARTICLES: SYNTHESIS AND APPLICATIONS
8 Synthesis of Semiconductor Nanoparticles
9 Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature
10 Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods
11 In Vivo， Site-Specific， Covalent Conjugation of Quantum Dots to Proteins via Split-Intein Splicing
12 Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis
13 The Use of Quantum Dots for Immunochemistry Applications 185
14 Quantum Dots for Labeling Live Cells
15 Labeling and Imaging Mesenchymal Stein Cells with Quantum Dots
16 The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption， Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
PART III MAGNETIC NANOPARTICLES: SYNTHESIS AND APPLICATIONS
17 Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging 221
18 Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging 239
19 Noninvasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry 253
20 Synthesis， Application， and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants 263
PART IV ORGANIC~ POLYMERIC， BIOMIMETIC， AND BIOCOMPATIBLE NANOPARTICLES AND THEIR APPLICATIONS
21 Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots ("Jelly Dots") 275
22 Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery
23 Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery 295
24 Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases
25 Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulation Efficiency
26 Synthesis of Poly (2-Hydroxyethyl Methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications 321
27 Preparation of Lipid: Peptide: DNA (LPD) Nanoparticles and Their Use for Gene Transfection
28 Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications.
29 Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins
30 Synthesis， Surface Modification， Characterization， and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles
31 Extraction of Organic Nanoparticles from Plants
PAKT V NANO-TOXICITY AND NANO-SAFETY
32 Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4，5-Dimethylthiazol-2-yl)-5-( 3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay 395
33 Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression 403
34 Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells
35 Assessment of Toxicity of Nanoparticles Using Insects as Biological Models
36 Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues 435
PART VI NANOPARTICLES CHARACTERIZATION
37 Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles 453
38 Total Internal Reflection Fluorescence (TIRF) Microscopy for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction 473
39 Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells 483
40 Detection ofTiO2 Nanoparticles in Cells by Flow Cytometry 497
41 Nanoparticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions 511
42 Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis 525
43 Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules 535
