Amyloid proteins : methods and protocols / edited by Einar M. Sigurdsson, Miguel Carelo, Maria Gasset. — 2nd ed. — New York : Humana Press ; Springer, c2012. – (58.17/M592/v.849)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I IN VITRO MODELS AND ASSAYS
    1 Rapid Generation of Dityrosine Cross-linked Aβ Oligomers via Cu-Redox Cycling 3
    2 Application of Photochemical Cross-linking to the Study of Oligomerization of Amyloidogenic Proteins 11
    3 Preparation of Stable Amyloid [3-Protein Oligomers of Defined Assembly Order 23
    4 Purification and Fibrillation of Full-Length Recombinant PrP
    5 Featuring Amyloids with Fourier Transform Infrared and Circular Dichroism Spectroscopies
    6 Quasielastic Light Scattering Study of Amyloid J3-Protein Fibrillogenesis 69
    7 Conformations of Microtubule-Associated Protein Tau Mapped by Fluorescence Resonance Energy Transfer 85
    8 Measuring the Kinetics of Amyloid Fibril Elongation Using Quartz Crystal Microbalances 101
    9 X-Ray Fibre Diffraction Studies of Amyloid Fibrils
    10 Structural Characterization of Prefibrillar Intermediates and Amyloid Fibrils by Small-Angle X-Ray Scattering 137
    11 Atomic Force Fluorescence Microscopy in the Characterization of Amyloid Fibril Assembly and Oligomeric Intermediates 157
    12 Investigating Fibrillar Aggregates of Tau Protein by Atomic Force Microscopy
    13 Structural Studies of Amyloids by Quenched Hydrogen-Deuterium Exchange by NMR
    14 Cyclic Amplification of Prion Protein Misfolding
    15 Search for Amyloid-Binding Proteins by Affinity Chromatography
    PART II CELL CULTURE MODELS AND ASSAYS
    16 Establishing the Links Between A[3 Aggregation and Cytotoxicity In Vitro Using Biophysical Approaches
    17 Preparation of Cultured Human Vascular Cells
    18 Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells from Mouse Brain 261
    19 In Vitro Assays Measuring Protection by Proteins such as Cystatin C of Primary Cortical Neuronal and Smooth Muscle Cells
    20 Study of Neurotoxic Intracellular Calcium Signalling Triggered by Amyloids
    21 Bacterial Amyloids
    22 Study of Amyloids Using Yeast
    23 Cell-to-Cell Transmission of α-Synuclein Aggregates
    PART III IN VIVO MODELS AND ASSAYS
    24 Subcutaneous Adipose Tissue Biopsy for Amyloid Protein Studies
    25 Analysis of S 100 Oligomers and Amyloids
    26 S100A8/A9 Amyloidosis in the Ageing Prostate: Relating Ex Vivo and In Vitro Studies
    27 Isolation of Amyloid by Solubilization in Water
    28 Histological Staining of Amyloid and Pre-amyloid Peptides and Proteins in Mouse Tissue
    29 A Pentameric Luminescent-Conjugated Oligothiophene for Optical Imaging of In Vitro-Formed Amyloid Fibrils and Protein Aggregates in Tissue Sections 425
    30 In Vivo Magnetic Resonance imaging of amyloid-β Plaques in Mice
    31 The Mouse Model for Scrapie: Inoculation， Clinical Scoring， and Histopathological Techniques
    32 Biochemical Isolation of Insoluble Tau in Transgenic Mouse Models of Tauopathies
    33 Tissue Processing Prior to Analysis of Alzheimer's Disease Associated Proteins and Metabolites， Including Aβ
    34 Aβ Measurement by Enzyme-Linked Immunosorbent Assay
    35 Cognitive and Sensorimotor Tasks for Assessing Functional Impairments in Mouse Models of Alzheimer's Disease and Related Disorders
    Index 541