Lipases and phospholipases : methods and protocols / edited by Georgina Sandoval. — New York : Humana Press, c2012. – (58.17/M592/v.861)

Contents

    Contents
    
    Contributors
    PART I OVERVIEW OF LIPASES， PHOSPHOHPASES AND RELATED ENZYMES
    1 Lipases: An Overview
    2 Lipases or Esterases: Does It Really Matter? Toward a New Bio-Physico-Chemical Classification
    3 Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillusflexus
    4 Phospholipases: An Overview
    PART II SCREENING AND SOURCES OF LIPASES， PHOSPHOLIPASES AND ITS INHIBITORS
    5 High-Throughput Screening Method for Lipases/Esterases
    6 Functional-Based Screening Methods for Lipases， Esterases， and Phospholipases in Metagenomic Libraries
    7 Plant Lipases: Partial Purification of Carica papaya Lipase
    8 Plant Phospholipases: An Overview
    9 Lipase and Phospholipase Inhibitors: Design and Applications
    10 High-Throughput Assay of Secreted Phospholipases A2 Inhibitors
    PART III CLONING， PRODUCTION， PURIFICATION AND CHARACTERIZATION OF LIPASES AND PHOSPHOLIPASES
    11 Heterologous Expression Systems for Lipases: A Review
    12 Streptomyces Phospholipase D Cloning and Production
    13 Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses
    14 Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite
    15 Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27
    16 Purification， Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase
    17 Phospholipase A2 Purification and Characterization: A Case Study
    PART IV OPTIMIZATION OF THE BIOCATALYST AND REACTION CONDITIONS
    18 The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Enzymes
    19 Molecular Modeling of Lipase Binding to a Substrate-Water Interface
    20 Different Strategies for Hyperactivation of Lipase Biocatalysts
    21 Rational Design of Immobilized Lipases and Phospholipases
    22 Thermodynamical Methods for the Optimization of Lipase-Catalyzed Reactions
    PART V APPLICATIONS OF LIPASES AND PHOSPHOLIPASES
    23 Lipases as Biocatalysts for the Synthesis of Structured Lipids
    24 Lipase-Catalyzed Modification of Phenolic Antioxidants
    25 Lipases as Catalysts in Synthesis of Fine Chemicals
    26 Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides
    27 Lipases as Biocatalyst for Biodiesel Production
    28 Synthesis of Biodegradable Polymers Using Biocatalysis with Yarrowia lipolytica Lipase
    29 Phospholipases in Food Industry: A Review
    30 Lipase and Phospholipase Biosensors: A Review
    Index