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Primary and stem cells : gene transfer technologies and applications / edited by Uma Lakshmipathy and Bhaskar Thyagarajan. — Hoboken, N.J. : John Wiley and Sons, c2012. – (58.22/P952) |
Contents
CONTENTS
FOREWORD
PREFACE
CONTRIBUTORS
PART I: CLONING AND GENE DELIVERY
1. DNA Assembly Technologies Based on Homologous Recombination
2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells
3. Strategies for the Delivery of Naked DNA
PART II NONINTEGRATING TECHNOLOGIES
4. Episomal Vectors
5. Nonintegrating DNA Virus
6. Nonintegrating RNA Viruses
7. Protein Delivery
PART III: INTEGRATING TECHNOLOGIES
8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery
9. Integrating Viral Vectors for Gene Modifications
10. Bacteriophage Integrases for Site-Specific Integration
11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells
PART IV: APPLICATIONS
12. Modified Stem Cells as Disease Models and in Toxicology Screening
13. Screening and Drug Discovery
INDEX