Chemical genomics and proteomics : reviews and protocols / edited by Edward D. Zanders. — New York, N.Y. : Humana Press, c2012. – (58.17/M592/v.800)

Contents

    Contents
    
    Contributors
    PART I REVIEWS
    1 Overview of Chemical Genomics and Proteomics
    2 Exploring Chemical Space: Recent Advances in Chemistry
    3 In Silico Design of Small Molecules
    4 Surface Plasmon Resonance for Proteomics
    PART II PROTOCOLS
    5 Biomimetic Affinity Ligands for Immunoglobulins Based on the Multicomponent Ugi Reaction 57
    6 Preparation of Photo-Cross-Linked Small Molecule Affinity Matrices for Affinity Selection of Protein Targets for Biologically Active Small Molecules
    7 Profiling Cellular Myristoylation and Palmitoylation Using ω-Alkynyl Fatty Acids
    8 Fluorescence Labels in Kinases: A High-Throughput Kinase Binding Assay for the Identification of DFG-Out Binding Ligands
    9 Electrochemical Aptamer Sensor for Small Molecule Assays
    10 Miniaturized， Microarray-Based Assays for Chemical Proteomic Studies of Protein Function 133
    11 Glycan Microarrays 163
    12 Resolving Bottlenecks for Recombinant Protein Expression in E. coli 173
    13 Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System
    14 Systems for the Cell-Free Synthesis of Proteins
    15 Protein Identification by MALDI-TOF Mass Spectrometry 227
    Index 241