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Ribosome display and related technologies : methods and protocols / edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805) |
Contents
Contents
Preface
Contributors
PART I REVIEW
1 Ribosome Display: A Perspective
PART II TRANSLATION EXTRACT PREPARATION
2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts
PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS
3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 45
4 Stabilized Ribosome Display for In Vitro Selection
5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75
6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 87
7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101
8 cDNA Display: Rapid Stabilization of mRNA Display 113
PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS
9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis
10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display
11 Evolution of Protein Stability Using Ribosome Display
12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries
13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display
14 Peptide Screening Using PURE Ribosome Display
15 Rapid Selection of High-Affinity Binders Using Ribosome Display 261
16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287
17 Identification of Candidate Vaccine Genes Using Ribosome Display 299
18 Ribosome Display for the Selection of Sac7d Scaffolds 315
PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS
19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335
20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349
21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 367
PART VI CASE STUDIES
22 Optimization of CAT-354， a Therapeutic Antibody Directed Against Interleukin-13， Using Ribosome Display
23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 403
Index 423
