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Chemical proteomics : methods and protocols / edited by Gerard Drews and Marcus Bantscheff. — New York : Humana Press, c2012. – (58.17/M592/v.803) |
Contents
Contents
Preface
Contributors
PART I INTRODUCTION
1 Mass Spectrometry-Based Chemoproteomic Approaches
2 Chemical Proteomics in Drug Discovery
PART II SMALL MOLECULES AND PROBE DESIGN
3 Compound Immobilization and Drug-Affinity Chromatography 25
4 Affinity-Based Chemoproteomics with Small Molecule-Peptide Conjugates 39
5 A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine 55
6 Probing Proteomes with Benzophenone Photoprobes
7 Biotinylated Probes for the Analysis of Protein Modification by Electrophiles
8 Profiling of Methyltransferases and Other S-Adenosyl-L-Homocysteine-Binding Proteins by Capture Compound Mass Spectrometry 97
PART III TARGET DISCOVERY AND TARGET VALIDATION
9 Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC
10 Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags 141
11 Affinity-Based Profiling of Dehydrogenase Subproteomes 157
12 Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics 167
13 Fluorescence-Based Proteasome Activity Profiling 183
14 Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions 205
15 Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry 219
16 Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies 231
PART IV LIGAND DISCOVERY
17 Ligand Discovery Using Small-Molecule Microarrays 249
18 Working with Small Molecules: Preparing and Storing Stock Solutions and Determination of Kinetic Solubility 265
19 A Database for Chemical Proteomics: ChEBI 273
20 Working with Small Molecules: Rules-of-Thumb of "Drug Likeness" 297
Index 309
