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Neurodegeneration : methods and protocols / edited by Giovanni Manfredi, Hibiki Kawamata. — New York : Humana Press, C2011. – (58.17/M592/v.793) |
Contents
Contents
PART I NEURODEGENERATION
1 Introduction to Neurodegenerative Diseases and Related Techniques
2 In Vivo and In Vitro Determination of Cell Death Markers in Neurons
3 Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods
PART II NEURAL CELL CULTURE TECHNIQUES
4 Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex
5 Isolation and Culture of Postnatal Spinal Motoneurons
6 Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration 87
7 Neural Differentiation of Induced Pluripotent Stem Cells
PART III MODELS OF NEURODEGENERATION
8 Developing Yeast Models of Human Neurodegenerative Disorders 113
9 Modeling Dopamine Neuron Degeneration in Caenorhabditis elegans 129
10 Role of the Proteasome in Fly Models of Neurodegeneration 149
11 Modeling Neurodegenerative Diseases in Zebrafish Embryos
12 Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases
13 Modeling Focal Cerebral Ischemia In Vivo 195
14 Pharmacological Models of Parkinson's Disease in Rodents 211
15 Behavioral Phenotyping of Mouse Models of Neurodegeneration
PART IV NEURODEGENERATIVE DISEASE MECHANISMS: PROTEINOPATHIES
16 Characterization of Amyloid Deposits in Neurodegenerative Diseases 241
17 Detection of Protein Aggregation in Neurodegenerative Diseases
18 Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures
PART V NEURODEGENERATIVE DISEASE MECHANISMS: MITOCHONDRIA
19 Measurements of Threshold of Mitochondrial Permeability Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium
20 Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue 311
21 Monitoring Mitophagy in Neuronal Cell Cultures 325
PART VI NEURODEGENERATIVE DISEASE MECHANISMS: BIOLOGICAL FUNCTIONS
22 Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging 343
23 Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices
24 Imaging Presynaptic Exocytosis in Corticostriatal Slices
25 Microscopic Imaging of Intracellular Calcium in Live Cells Using Lifetime-Based Ratiometric Measurements of Oregon Green BAPTA-1
26 Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice 391
27 Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD 401
28 Designing， Performing， and Interpreting a Microarray-Based Gene Expression Study
PART VII THERAPEUTIC APPROACHES IN NEURODEGENERATION
29 Adeno-Associated Viral Gene Delivery in Neurodegenerative Disease
30 Specific Gene Silencing Using RNAi in Cell Culture
31 Stem Cell Transplantation for Spinal Cord Neurodegeneration
32 Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases
Index
