Cancer cell culture : methods and protocols / edited by Ian A. Cree. — 2nd ed. — New York : Humana Press, c2011. – (58.17/M592/v.731)

Contents

    Contents
    
    Preface
    Contributors
    1 Cancer Biology
    2 Principles of Cancer Cell Culture
    3 Storage of Cell Lines 27
    4 Characterization and Authentication of Cancer Cell Lines: An Overview 35
    5 Online Verification of Human Cell Line Identity by STR DNA Typing 45
    6 Cytogenetic Analysis of Cancer Cell Lines 57
    7 Cell Culture Contamination
    8 Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
    9 Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics
    10 Quality Assurance and Good Laboratory Practice
    11 Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning 125
    12 Isolation and Culture of Colon Cancer Cells and Cell Lines
    13 Isolation and Culture of Melanoma and Naevus Cells and Cell Lines 141
    14 Isolation and Culture of Squamous Cell Carcinoma Lines 151
    15 Isolation and Culture of Ovarian Cancer Cells and Cell Lines 161
    16 Establishment and Culture of Leukemia-Lymphoma Cell Lines 181
    17 Isolation of Inflammatory Cells from Human Tumours 201
    18 Isolation of Endothelial Cells from Human Tumors
    19 Cellular Chemosensitivity Assays: An Overview
    20 Cell Sensitivity Assays: The MTT Assay 237
    21 Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay
    22 Differential Staining Cytotoxicity Assay: A Review
    23 Real-Time Cytotoxicity Assays 285
    24 Purification of Annexin V and Its Use in the Detection of Apoptotic Cells 293
    25 Measurement of DNA Damage in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay 309
    26 Molecular Breakpoint Analysis of Chromosome Translocations in Cancer Cell Lines by Long Distance Inverse-PCR 321
    27 Cell Migration and Invasion Assays 333
    28 Angiogenesis Assays
    29 Flow Cytometric DNA Analysis of Human Cancers and Cell Lines
    30 Expression Analysis of Homeobox Genes in Leukemia/Lymphoma Cell Lines 371
    31 Measuring Gene Expression from Cell Cultures by Quantitative Reverse-Transcriptase Polymerase Chain Reaction
    32 Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins
    33 Development of Rituximab-Resistant B-NHL Clones: An In Vitro Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated Immunotherapy 407
    34 Analysis of Drug Interactions
    35 Transfection and DNA-Mediated Gene Transfer 435
    36 Drug Design and Testing: Profiling of Antiproliferative Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]- Thymidine Incorporation Assay 451
    37 Feeder Layers: Co-culture with Nonneoplastic Cells 467
    38 Xenotransplantation of Breast Cancers
    Appendix A
    Appendix B
    Index