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Claudins : methods and protocols / edited by Kursad Turksen. — New York : Humana Press, c2011. – (58.17/M592/v.762) |
Contents
Contents
Preface
Contributors
1 Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling
2 Biochemical Analysis of Claudin-Binding Compatibility
3 Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines
4 The Tight Junction, Intercellular Seal as a Cell Signaling Player Protocols for Examination of Its Status
5 Interactions Between Clostridium perfringens Enterotoxin and Claudins 63
6 Biophysical Methods to Probe Claudin-Mediated Adhesion at the Cellular and Molecular Level
7 Detection of Tight Junction Barrier Function In Vivo by Biotin
8 The Coculture Method to Examine Interactions Between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells
9 Claudin-4: Functional Studies Beyond the Tight Junction
10 Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin-16
11 Claudin Family Proteins in Caenorhabditis elegans
12 In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka
13 Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium
14 Manipulating Claudin Expression in Avian Embryos
15 Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues 213
16 Expression and Function of Claudins in Hepatocytes
17 Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture
18 Development of Biological Tools to Study Claudins in the Male Reproductive Tract
19 Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo 275
20 Laboratory Methods in the Study of Endometrial Claudin-4 281
21 Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction
22 Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells 303
23 Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier
24 MMP-Mediated Disruption of Claudin-5 in the Blood-Brain Barrier of Rat Brain After Cerebral Ischemia 333
25 Claudin-5 Expression in In Vitro Models of the Blood-Brain Barrier 347
26 HIV- 1-Induced Alterations of Claudin-5 Expression at the Blood-Brain Barrier Level 355
27 Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Biopsies 371
28 Claudin-16/Paracellin-1, Cloning, Expression, and Its Role in Tight Junction Functions in Cancer and Endothelial Cells 383
29 Dynamics of Claudins Expression in Colitis and Colitis-Associated Cancer in Rat
30 Anti-claudin-4-Conjugated Highly Luminescent Nanoparticles as Biological Labels for Pancreatic Cancer Sensing 427
Index 439