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Plant transcription factors : methods and protocols / edited by Ling Yuan, Sharyn E. Perry. — New York : Humana Press, c2011. – (58.17/M592/v.754) |
Contents
Contents
SECTION I: INTRODUCTION
1. MADS and More: Transcription Factors That Shape the Plant
SECTION II: IDENTIFICATION OF TRANSCRIPTION FACTORS
2. In Silico Mining and PCR-Based Approaches to Transcription Factor Discovery in Non-model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers
3. Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System 45
4. A Transposon-Based Activation Tagging System for Gene Function Discovery in Arabidopsis
SECTION III: FUNCTIONAL VERIFICATION
5. CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors
6. Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts
7. Microarray-Based Identification of Transcription Factor Target Genes
SECTION IV: PROTEIN-PROTEIN INTERACTION AND OTHER MEANS FOR ACTIVITY CONTROL
8. Yeast Protein-Protein Interaction Assays and Screens
9. Mapping Functional Domains of Transcription Factors 167
10. Bimolecular Fluorescence Complementation as a Tool to Study Interactions of Regulatory Proteins in Plant Protoplasts 185
11. Isolation of Transcription Factor Complexes from Arabidopsis Cell Suspension Cultures by Tandem Affinity Purification 195
12. Assaying Transcription Factor Stability
13. How to Assess the Intercellular Trafficking of Transcription Factors 235
SECTION V: PROTEIN-DNA INTERACTION
14. SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a Powerful Tool for Deciphering the Protein-DNA Interaction Space 249
15. Footprinting and Missing Nucleoside Analysis of Transcription Factor-DNA Complexes
16. Chromatin Immunoprecipitation to Verify or to Identify In Vivo Protein-DNA Interactions
17. Visualizing and Characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors 293
18. Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method 307
SECTION VI: ENGINEERING METABOLIC PATHWAYS USING TRANSCRIPTION FACTORS
19. Directed Evolution Through DNA Shuffling for the Improvement and Understanding of Genes and Promoters
Index