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Methods in cell biology. v.102, Recent advances in cytometry. Part A : Instrumentation, methods / edited by Zbigniew Darzynkiewicz ... [et al]. — Amsterdam : Academic Press, c2011. – (58.1574/M592/v.102) |
Contents
CONTENTS
Contributors
Preface to fifth edition
PART A -- Instrumentation, Methods
1. Introduction A: Recent Advances in Cytometry Instrumentation, Probes, and Methods--Review
I. Preface
II. Image Cytometry
III. New Instrumentations
IV. New Probes, Components, and Methods
V. New Strategies for Data Analysis
VI. Perspective
References
Section I. Down-sizing cytometry to "micro" dimension
2. Droplet Microfluidics for High-throughput Analysis of Cells and Particles
I. Introduction
II. Droplet Microfluidics
III. Detection Techniques and Methodologies in Droplet Microfluidics
IV. High-Throughput Cell and Particle Analysis in Droplet Microfluidics
V. Perspectives
VI. Conclusions
References
3. Parallel Imaging Microfluidic Cytometer
I. Introduction
II. Background
III. Instrument Design
IV. Operating Methods
V. Results
VI. Conclusions
References
4. Microfluidic Systems for Live Cell Imaging
I. Introduction
II. Physical Properties of Microfluidic Cell Culture
III. Microfabrication Methods
IV. Flow Control
V. Design Aspects
VI. Example Applications
VII. Conclusion
References
5. Rise of the Micromachines: Microfluidics and the Future of Cytometry
I. Introduction
II. The Smaller the Better: Microfluidics and Enabling Prospects for Single Cytomics
III. Microflow Cytometry (μFCM)
IV. Microfluidic Cell Sorting 0.tFACS)
V. Real-Time Cell Analysis: Living Cell Microarrays and a Real-Time Physiometry on a Chip
VI. Conclusions
References
6. Label-Free Resistive-Pulse Cytometry
I. Introduction
II. Resistive-Pulse Sensing
III. Coulter Counter on a Chip
IV. Multiparametric RPS for Cell Cytometry
V. Device Fabrication and Experimental Methods
VI. Cell Size
VII. Cell-Surface Marker Screening
VIII. Applications
IX. Conclusion
References
Section II. Imaging cytometry
7. Laser Scanning Cytometry and Its Applications: A Pioneering Technology in the Field of Quantitative Imaging Cytometry
I. Introduction
II. Definition of Quantitative Imaging Cytometry (QIC) and Key Features Distinguishing Imaging Cytometry Platforms
III. Technical and Analytical Features of iGeneration Laser Scanning Cytometry
IV. Selected Application Areas of LSC
V. Concluding Remarks
References
8. Analytical Capabilities of the ImageStream Cytometer
I. Introduction
II. Background
III. Methods
IV. Applications of ImageStream System
V. Future Directions
References
9. Laser Scanning Cytometry: Capturing the Immune System In situ
I. Introduction
II. Background: Laser Scanning Cytometry Technology for Quantitatively Imaging and Analyzing Immune Responses In situ
III. Rationale for LSC Analysis of Antigen-Specific T cell Responses In vitro and In vivo
IV. Detailed Protocols for Tracking Antigen-specific T Cell Responses
V. Acquisition and Analysis of Data Using WinCyte Software
VI. Results: Analysis of the Role of pERK Signaling in Antigen-Specific Priming of T Cells
VII. Application of LSC Technology to Analysis of the Immune System in Health and Disease
VIII. Concluding Remarks and Future Directions
References
10. Image Cytometry Analysis of Circulating Tumor Cells
I. Introduction
II. Background and Technical Considerations
III. Image Cytometry: Methods and Results
IV. Conclusions and Future Directions
References
11. Preclinical Applications of Quantitative Imaging Cytometry to Support Drag Discovery
I. Introduction
II. Specific Examples: Example 1 - High-content Automated Tissue Analysis of ZDF Rat Pancreas
III. Example 2 - Analysis of Biomarkers in Tissue Microarrays
IV. Conclusions and Future Directions
References list
12. Leveraging Image Cytometry for the Development of Clinically Feasible Biomarkers: Evaluation of Activated Caspase-3 in Fine Needle Aspirate Biopsies
I. Introduction
II. Materials
III. Staining and Cytometric Analyses of FNAs or Culture Cell Lines
IV. Critical Aspects of the Procedure
V. Results and Discussion
VI. Biological Information and Future Directions
References
13. Automation of the Buccal Micronucleus Cytome Assay Using Laser Scanning Cytometry
I. Introduction
II. Rationale
III. Methods
IV. Summary
References
14. Laser Scanning Cytometry of Mitosis: State and Stage Analysis
I. Introduction
II. Background
III. Methods
IV. Discussion
References
Section III. Instrumentation, new probes and methods
15. Lasers in Flow Cytometry
I. Introduction
II. Laser Characteristics for Flow Cytometry
III. Laser Safety
IV. Laser Diodes
V. Diode-Pumped Solid State (DPSS) Lasers
VI. Lasers by Wavelength
VII. Multiwavelength Sources for Flow Cytometry
VIII. Summary
References
16. The Use of Hollow Fiber Membranes Combined with Cytometry in Analysis of Bacteriological Samples
I. Introduction
II. Assessment of Membrane Suitability For Encapsulation of Microorganisms
III. The Release of Bacteria Products
IV. Production and Release by Bacteria of Biologically Active Factor(s)
V. Conclusion
References
17. Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry
I. Introduction
II. Major Characteristics of FPs
III. Modem Advanced Red-Shifted FPs
IV. Simultaneous Detection of Multiple FPs
V. Fluorescent Timers
VI. FRET-Based Genetically Encoded Biosensors
VII. Biosensors Consisting of a Single FP
VIII. Perspectives
References
18. Quantum Dot Technology in Flow Cytometry
I. Introduction
II. Fundamental Aspects of QD Flow Cytometry: Fluorescence and Hardware
III. Utility of QDs in Multicolor Flow Cytometry
IV. QD Conjugation to Antibodies
V. Developing Staining Panels with QDs
VI. Troubleshooting QD Use
VII. Applications for QDs
VIII. Conclusion
References
19. Background-free Cytometry Using Rare Earth Complex Bioprobes
I. Introduction
II. Instrumentation Development
III. Bioprobes Development
IV. Conclusion
References
20. Surface-Enhanced Raman Scattering (SERS) Cytometry
I. Introduction
II. Multiparameter Fluorescence Measurements
III. Raman Scattering in Cytometry
IV. Reagents and Instrumentation
V. SERS Cytometry Applications
VI. Summary and Prospects
References
21. Recent Advances in Flow Cytometric Cell Sorting
I. Introduction
II. Single-Cell Deposition and Index Sorting
III. Positional Sorting
IV. Reflective Plate Sorting
V. Summary
References
Index