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Plant tissue culture, development, and biotechnology / edited by Robert N. Trigiano, Dennis J. Gray. — Boca Raton : CRC Press, c2011. – (58.838/P713d) |
Contents
Contents
Acknowledgments
Editors
Contributors
Chapter 1 Introduction 3
SECTION II Supporting Methodologies and General Concepts
Chapter 2 Getting Started with Tissue Culture--Media Preparation， Sterile Technique， and Laboratory Equipment
Chapter 3 Nutrition of Cell and Organ Cultures 27
Chapter 4 PGRs and Their Use in Micropropagation 33
Chapter 5 Elements of In Vitro Research 57
Chapter 6 Proper Use of Microscopes 75
Chapter 7 Plant Histological Techniques 99
Chapter 8 A Brief Introduction to Plant Anatomy and Morphology 111
Chapter 9 Seed Development and Germination 127
Chapter 10 Molecular Tools for Studying Plant Genetic Diversity 141
Chapter 11 Molecular Approaches to the Study of Plant Development 155
SECTION III Propagation and Development Concepts
Chapter 12 Propagation by Shoot Culture 181
Chapter 13 Micropropagation of Syngonium by Shoot Culture 193
Chapter 14 Micropropagation and In Vitro Flowering of Rose 201
Chapter 15 Micropropagation of Potato by Node Culture and Microtuber Production 207
Chapter 16 Commercial Laboratory Production 213
Chapter 17 Detection and Elimination of Microbial Endophytes and Prevention of Contamination in Plant Tissue Culture 223
Chapter 18 Culture Indexing for Bacterial and Fungal Contaminants 239
Chapter 19 Propagation from Nonmeristematic Tissues--Organogenesis 245
Chapter 20 Developing a Molecular Understanding of In Vitro and In Planta Shoot Organogenesis 259
Chapter 21 Direct Shoot Organogenesis from Leaf Explants of Chrysanthemum and African Violets 279
Chapter 22 Propagation from Nonmeristematic Tissues--Nonzygotic Embryogenesis 293
Chapter 23 Developmental and Molecular Aspects of Nonzygotic (Somatic) Embryogenesis 307
Chapter 24 Embryogenic Callus and Suspension Cultures from Leaves of Orchardgrass
Chapter 25 Direct Nonzygotic Embryogenesis from Leaves and Flower Receptacles of Cineraria 341
SECTION IV Crop Improvement Techniques
Chapter 26 Protoplasts--An Increasingly Valuable Tool in Plant Research 349
Chapter 27 Demonstration of Principles of Protoplast Isolation Using Chrysanthemum and Orchardgrass Leaves 365
Chapter 28 Isolation， Culture， and Fusion of Tobacco and Potato Protoplasts 373
Chapter 29 Haploid Cultures 385
Chapter 30 Production of Haploid Tobacco and Potato Plants Using Anther Culture 397
Chapter 31 Embryo Rescue 405
Chapter 32 Promoters and Gene Expression Regulation 413
Chapter 33 Genetic Engineering Technologies 423
Chapter 34 Transformation of Plant Meristems 435
Chapter 35 Genetic Transformation of Chysanthemum and Tobacco Using Agrobacterium tumefaciens 453
Chapter 36 Genetic Transformation of Tobacco and Production of Transgenic Plants
Chapter 37 Genetically Modified Controversies 473
Chapter 38 Cryopreservation of Plant Cells， Tissues， and Organs 489
Chapter 39 Cryopreservation of In Vitro Grown Shoot Tips 497
Chapter 40 Cryopreservation of Orthodox and Recalcitrant Seed 507
Chapter 41 Plant Biotechnology for the Production of Natural Products 515
Chapter 42 Pigment Production in Ajuga Cell Cultures 531
Chapter 43 Variation in Tissue Culture 543
SECTION V The Business of Biotechnology
Chapter 44 Biotechnology Entrepreneurship in the 21st Century
Chapter 45 Intellectual Property Protection for Plants 563
Index 573
