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Cell migration : developmental methods and protocols / edited by Claire M. Wells, Maddy Parsons. — 2nd ed. — New York : Humana Press, c2011. – (58.17/M592/v.769) |
Contents
Preface
Contributors
1 Cell Migration: An Overview
2 Scratch-Wound Assay 25
3 HGF-Induced DU145 Cell Scatter Assay 31
4 Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
5 Imaging Cells Within 3D Cell-Derived Matrix 53
6 Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro
7 Boyden Chamber 87
8 Transwell Invasion Assays 97
9 Imaging Podosome Dynamics and Matrix Degradation 111
10 Endothelial Cell Migration Under Flow 137
11 In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments
12 Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy
13 Chemotaxis of Slow Migrating Mammalian Cells Analysed by Video Microscopy
14 Live Cell Fluorescence Microscopy Techniques 205
15 Measuring Invasion in an Organotypic Model 223
16 Analysis of Cell Migration Using Caenorhabditis elegans as a Model System 233
17 Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration
18 Measuring Inflammatory Cell Migration in the Zebrafish
19 Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement 277
20 Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants
21 Simple Experimental and Spontaneous Metastasis Assays in Mice
22 Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour of Cancer Cells In Vivo 331
23 Measuring Angiogenesis in Mice
24 Time-Lapse Imaging of Chick Cardiac Precursor Cells .
25 Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy
26 Fluorescence Recovery After Photobleaching
27 Measuring FRET Using Time-Resolved FLIM
28 Cell Migration in Confinement: A Micro-Channel-Based Assay
29 Functional Screening with a Live Cell Imaging-Based Random Cell Migration Assay
30 Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays 449
Index 461