DNA recombination : methods and protocols / edited by Hideo Tsubouchi. — New York : Humana, c2011. – (58.17/M592/v.745)

Contents

    Contents
    
    Preface
    Contributors
    SECTION I: GENETIC AND MOLECULAR BIOLOGICAL APPROACHES WITH YEAST
    1. Methods to Study Mitotic Homologous Recombination and Genome Stability
    2. Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends
    3. Characterization of Meiotic Recombination Initiation Sites Using Pulsed-Field Gel Electrophoresis
    4. Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-Stranded DNA
    5. Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes
    6. Molecular Assays to Investigate Chromatin Changes During DNA Double-Strand Break Repair in Yeast
    7. Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis
    8. Mapping of Crossover Sites Using DNA Microarrays
    9. Using the Semi-synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase， Mek1
    10. Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae
    11. In Vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae
    12. Detection of RNA-Templated Double-Strand Break Repair in Yeast 193
    SECTION II: GENETIC AND MOLECULAR BIOLOGICAL APPROACHES WITH HIGHER EUKARYOTES
    13. SNP-Based Mapping of Crossover Recombination in Caenorhabditis elegans
    14. Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana
    15. Isolation of Meiotic Recombinants from Mouse Sperm 251
    16. Homologous Recombination Assay for Interstrand Cross-Link Repair 283
    17. Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line， DT40
    18. Understanding the Immunoglobulin Locus Specificity of Hypermutation
    SECTION III: IN VITRO RECONSTITUTION OF HOMOLOGOUS RECOMBINATION REACTIONS AND SINGLE MOLECULAR ANALYSIS OF RECOMBINATION PROTEINS
    19. Quality Control of Purified Proteins Involved in Homologous Recombination
    20. Assays for Structure-Selective DNA Endonucleases
    21. In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
    22. An In Vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by a Eukaryotic Recombinase
    23. Reconstituting the Key Steps of the DNA Double-Strand Break Repair In Vitro
    24. Biochemical Studies on Human Rad5I-Mediated Homologous Recombination
    25. Studying DNA Replication Fork Stability in Xenopus Egg Extract
    26. Supported Lipid Bilayers and DNA Curtains for High-Throughput Single-Molecule Studies
    27. FRET-Based Assays to Monitor DNA Binding and Annealing by Rad52 Recombination Mediator Protein
    28. Visualization of Human Dmc1 Presynaptic Filaments
    SECTION IV: CELL BIOLOGICAI， APPROACHES TO STUDY THE IN VIVO BEHAVIOR OF HOMOLOGOUS RECOMBINATION
    29. Tracking of Single and Multiple Genomic Loci in Living Yeast Cells
    30. Cell Biology of Homologous Recombination in Yeast
    31. Live Cell Imaging of Meiotic Chromosome Dynamics in Yeast
    32. Chromosome Structure and Homologous Chromosome Association During Meiotic Prophase in
    Index