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Mesenchymal stem cell assays and applications / edited by Mohan C. Vemuri, Lucas G. Chase, Mahendra S. Rao. — Totowa, N.J. : Humana, 2011. – (58.17/M592/v.698) |
Contents
Contents
PART I INTRODUCTION
1 Mesenchymal Stem Cell Assays and Applications
PART II ISOLATION AND EXPANSION OF MSCs FROM VAIUOUS SOURCES
2 Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow.
3 Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
4 Isolation and Growth of Adipose Tissue-Derived Stem Cells
5 Isolation， Culture， and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells
6 The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions
7 Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture
8 Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells
9 A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells
10 Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas
11 Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
12 Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells
13 Cryopreservation and Revival of Mesenchymal Stromal Cells
14 Dynamic Expansion Culture for Mesenchymal Stem Cells
15 Ex Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers
PART III MSC LINEAGE DIFFERENTIATION AND ANALYSIS
16 Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells 201
17 Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells
18 Bioreactor Cultivation of Functional Bone Grafts 231
19 Adipogenic Differentiation of Human Mesenchymal Stem Cells 243
20 Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks 253
21 Use of Human Mesenchymal Stem Cells as Alternative Source of Smooth Muscle Cells in Vessel Engineering 279
22 Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
23 Hepatic Differentiation of Mesenchymal Stem Cells: In Vitro Strategies 305
24 Hepatic Transplantation of Mesenchymal Stem Cells in Rodent Animal Models 315
25 Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells
PART IV MSC PHENOTYPIC CHARACTERIZATION AND EXTENDED APPLICATIONS
26 Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic， Osteogenic， and Adipogenic Lineages 353
27 Panel Development for Multicolor Flow-Cytometry Testing of Proliferation and Immunophenotype in hMSCs 367
28 Simplified PCR Assay for Detecting Early Stages of Multipotent Mesenchymal Stromal Cell Differentiation
29 Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins 405
30 Comparison of Microarray and Quantitative Real-Time PCR Methods for Measuring MicroRNA Levels in MSC Cultures.
31 Two Dimensional Gel Electrophoresis Analysis of Mesenchymal Stem Cells 431
32 Proteomic Analysis of Human Mesenchymal Stem Cells 443
33 Metabolic Labeling and Click Chemistry Detection of Glycoprotein Markers of Mesenchymal Stem Cell Differentiation 459
34 BacMam-Mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells 485
35 Cell Surface Engineering of Mesenchymal Stem Cells 505
Index 525
