Serum/plasma proteomics : methods and protocols / edited by Richard J. Simpson and David W. Greening. — New York : Humana, c2011. -- (58.17/M592/v.728)

Contents

    CONTENTS
    
    Preface
    Contributors
    PART I FRACTIONATION STRATEGIES FOR IN-DEPTH BLOOD PROTEOME ANALYSIS
    1 Plasma Biomarker Discovery Using 3D Protein Profiling Coupled with Label-Free Quantitation
    2 Intact Protein Separation by One- and Two-Dimensional Liquid Chromatography for the Comparative Proteomic Separation of Partitioned Serum or Plasma
    3 In-Depth Analysis of a Plasma or Serum Proteome Using a 4D Protein Profiling Method
    4 Intact-Protein Analysis System for Discovery of Serum-Based Disease Biomarkers
    5 Model-Based Discovery of Circulating Biomarkers
    6 Low-Molecular Weight Plasma Proteome Analysis Using Centrifugal Ultrafiltration
    7 High-Throughput Analysis of Glycoproteins from Plasma
    PART II BLOOD COLLECTION AND HANDLING STRATEGIES
    8 Minimizing Preanalytical Variation of Plasma Samples by Proper Blood Collection and Handling 137
    9 Collection and Handling of Blood Specimens for Peptidomics 151
    10 Investigation of Peptide Biomarker Stability in Plasma Samples Using Time-Course MS Analysis
    PART III METHODS FOR QUANTITATIVE ASSAYING OF BLOOD-BASED BIOMARKERS
    11 Biomarker Validation in Blood Specimens by Selected Reaction Monitoring Mass Spectrometry of N-Glycosites
    12 A Fluorescent Microsphere-Based Method for Assay of Multiple Analytes in Plasma
    13 Immuno-Mass Spectrometry: Quantification of Low-Abundance Proteins in Biological Fluids 207
    14 Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with laC6-Reducing Sugars 219
    PART IV PROTEOME ANALYSIS OF BLOOD CELL COMPONENTS， VESICLES， AND BLOOD-RELATED FLUIDS
    15 Exosome Isolation for Proteomic Analyses and RNA Profiling
    16 Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid 247
    17 A Protocol for the Preparation of Cryoprecipitate and Cryodepleted Plasma
    18 Preparation of Platelet Concentrates 267
    19 Phosphoproteome Analysis of the Platelet Plasma Membrane 279
    PART V BIOINFORMATIC ANALYSIS OF BLOOD PROTEINS AND PEPTIDES
    20 Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease 293
    21 Data Management in Mass Spectrometry-Based Proteomics 321
    22 Bioinformatics Challenges in the Proteomic Analysis of Human Plasma 333
    23 Using the Human Plasma Peptide Atlas to Study Human Plasma Proteins 349
    Appendix 375
    Index 395