Mammalian chromosome engineering : methods and protocols / edited by Gyula Hadlaczky. — New York : Humana, c2011. – (58.17/M592/v.738)

Contents

    CONTENTS
    
    1 Developing Extrachromosomal Gene Expression Vector Technologies: An Overview
    2 High Capacity Extrachromosomal Gene Expression Vectors
    3 Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview
    4 Chromosome Transfer Via Cell Fusion
    5 Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview
    6 Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System
    7 Construction and Use of a Bottom-Up HAC Vector for Transgene Expression
    8 De Novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification 111
    9 Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes 127
    10 Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System
    11 Dendrimer Mediated Transfer of Engineered Chromosomes
    12 Engineered Chromosomes in Transgenics
    13 Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System 183
    14 Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview
    15 Engineered Mammalian Chromosomes in Cellular Protein Production Future Prospects
    Index