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Antiviral RNAi : concepts, methods, and applications / edited by Ronald P. van Rij. — [Totowa, N.J.] : Humana, c2011. – (58.17/M592/v.721) |
Contents
CONTENTS
Preface
Contributors
PART I GENERAL INTRODUCTION
1 Defense and Counterdefense in the RNAi-Based Antiviral Immune System in Insects
2 RNAi and Cellular miRNAs in Infections by Mammalian Viruses 23
3 Viral miRNAs
4 Progress in RNAi-Based Antiviral Therapeutics
5 Chemical Modification of Small Interfering RNA
PART II VIRAL SMALL RNAs
6 Viral Small RNA Cloning and Sequencing
7 Visitor， An Informatic Pipeline for Analysis of Viral siRNA Sequencing Datasets
8 Computational Prediction of Viral miRNAs
9 Detection of Viral microRNAs by Northern Blot Analysis 153
10 Detection of Viral microRNA with S 1 Nuclease Protection Assay 173
11 Characterization of RISC-Associated Adenoviral Small RNAs
PART III RNAI-BASED ANTIVIRAL DEFENSE
12 Identification of Viral Suppressors of RNAi by a Reporter Assay in Drosophila $2 Cell Culture
13 Dicer Assay in Drosophila $2 Cell Extract
14 Slicer Activity in Drosophila melanogaster $2 Extract 231
15 Gel Mobility Shift Assays for RNA Binding Viral RNAi Suppressors 245
16 dsRNA Uptake in Adult Drosophila
PART IV ANTIVIRAL RNAI THERAPY
17 Design of Small Interfering RNAs for Antiviral Applications 267
18 RNAi-Inducing Lentiviral Vectors for Anti-HIV-1 Gene Therapy 293
19 Production of Multicopy shRNA Lentiviral Vectors for Antiviral Therapy 313
20 Intranasal Delivery of Antiviral siRNA
21 Antibody-Mediated Delivery of siRNAs for Anti-HIV Therapy
22 Aptamer-Targeted RNAi for HIV-1 Therapy
PART V RNAI SCREENS TO STUDY VIRUS-HOST INTERACTIONS
23 RNAi Screening for Host Factors Involved in Viral Infection Using Drosophila Cells
24 Genome-Wide RNAi Screen for Viral Replication in Mammalian Cell Culture
25 RNAi Screening in Mammalian Cells to Identify Novel Host Cell Molecules Involved in the Regulation of Viral Infections 397
Index 407
