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Plant embryo culture : methods and protocols / edited by Trevor A. Thorpe and Edward C. Yeung. — [Totowa, N.J.] : Humana, c2011. – (58.17/M592/v.710) |
Contents
CONTENTS
PART I CULTURE OF ZYGOTIC EMBRYOS: FUNDAMENTAL STUDIES
1 Zygotic Embryo Culture: An Overview
2 In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture
3 Canola Zygotic Embryo Culture
4 Immature Seeds and Embryos of Medicago truncatula Cultured In vitro
5 In vitro Culture and Germination of Terrestrial Asian Orchid Seeds
6 In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos
PART II APPLICATION OF EMBRYO CULTURE TECHNIQUES: EMBRYO RESCUE
7 Immature Embryo Rescue and Culture
8 Chickpea Hybridization Using In vitro Techniques
9 Muskmelon Embryo Rescue Techniques using In vitro Embryo Culture 107
10 Phaseolus Immature Embryo Rescue Technology 117
11 Wide Crossing in Lentil through Embryo Rescue
12 Generation of Interspecific Hybrids of Trifolium Using Embryo Rescue Techniques
PART III CRYOPRESERVATION OF EMBRYOS
13 Cryopreservation of Embryos: An Overview
14 Cryogenic Technologies for the Long-Term Storage of Citrus Germplasm 185
15 Cryopreservation of Zygotic Embryonic Axes and Somatic Embryos of European Chestnut 201
16 Cryopreservation of Ilex Immature Zygotic Embryos
PART IV THE USE OF ZYGOTIC EMBRYOS AS EXPLANTS FOR SOMATIC EMBRYOGENESIS AND/OR ORGANOGENESIS
17 The Use of Zygotic Embryos as Explants for In Vitro Propagation: An Overview
18 Somatic Embryogenesis and Plant Regeneration in the Culture of Arabidopsis thaliana (L.) Heynh. Immature Zygotic Embryos
19 Pine Somatic Embryogenesis Using Zygotic Embryos as Explants 267
20 Micropropagation of Phalaenopsis Orchids via Protocorms and Protocorm-Like Bodies 293
PART V TRANSFORMATION USING ZYGOTIC EMBRYOS AS EXPLANTS
21 Genetic Transformation Protocols Using Zygotic Embryos as Explants: An Overview
22 Genetic Transformation Using Maize Immature Zygotic Embryos
23 Biolistic-Mediated Transformation Protocols for Maize and Pearl Millet Using Pre-Cultured Immature Zygotic Embryos and Embryogenic Tissue
24 Agrobacterium tumefaciens-Mediated Genetic Transformation of Cereals Using Immature Embryos
Index
