Metagenomics : methods and protocols / edited by Wolfgang R. Streit, Rolf Daniel. — New York : Humana ; London : Springer [distributor], c2010. – (58.17/M592/v.668)

Contents

    CONTENTS
    
    Preface
    Contributors
    1 Molecular Methods to Study Complex Microbial Communities
    2 Construction of Small-Insert and Large-Insert Metagenomic Libraries
    3 Construction and Screening of Marine Metagenomic Libraries
    4 Metagenomic Analysis of Isotopically Enriched DNA
    5 Wide Host-Range Cloning for Functional Metagenomics
    6 Cloning and Expression Vectors for a Gram-Positive Host， Streptomyces lividans
    7 Heterologous Gene Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus
    8 Novel Tools for the Functional Expression of Metagenomic DNA
    9 Screening of Functional Promoter from Metagenomic DNA for Practical Use in Expression Systems
    10 Substrate-Induced Gene Expression Screening: A Method for High-Throughput Screening of Metagenome Libraries
    11 Screens for Active and Stereoselective Hydrolytic Enzymes 169
    12 Screening for Cellulase-Encoding Clones in Metagenomic Libraries 177
    13 Screening Metagenomic Libraries for Laccase Activities 189
    14 Screening for N-AHSL-Based-Signaling Interfering Enzymes
    15 Identification of Molecular Markers to Follow Up the Bioremediation of Sites Contaminated with Chlorinated Compounds
    16 Methods for the Isolation of Genes Encoding Novel PHB Cycle Enzymes from Complex Microbial Communities
    17 Metagenomic Approaches to Identify and Isolate Bioactive Natural Products from Microbiota of Marine Sponges
    18 Screening for Novel Antibiotic Resistance Genes 265
    19 Novel Metal Resistance Genes from Microorganisms: A Functional Metagenomic Approach 273
    20 Retrieval of Full-Length Functional Genes Using Subtractive Hybridization Magnetic Bead Capture
    21 Detection and Isolation of Selected Genes of Interest from Metagenomic Libraries by a DNA Microarray Approach
    22 Application of DNA Microarray for Screening Metagenome Library Clones 313
    23 MetaGenomeThreader: A Software Tool for Predicting Genes in DNA-Sequences of Metagenome Projects
    Index