Protocols for neural cell culture / edited by Laurie C. Doering. — 4th ed. — Totowa, N.J. : Humana Press, c2010. – (59.455/P967/4th ed.)

Contents

    Contents
    
    Preface v
    Contributors ix
    1. Neurosphere and Neural Colony Forming Cell Assays
    2. Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells
    3. Culture and Differentiation of Human Neural Stem Cells
    4. Neural Differentiation of Human Embryonic Stem Cells
    5. Isolation and Culture of Primary Human CNS Neural Cells
    6. Bioengineering Protocols for Neural Precursor Cell Expansion
    7. Intracellular Calcium Assays in Dissociated Primary Cortical Neurons 125
    8. Dissociated Hippocampal Cultures
    9. Primary Sensory and Motor Neuron Cultures
    10. Retinal Cell and Tissue Culture
    11. Preparation of Normal and Reactive Astrocyte Cultures
    12. Oligodendrocyte Progenitor Cell Culture
    13. Isolation of Microglia Subpopulations
    14. Microglia from Progenitor Cells in Mouse Neopallium 241
    15. Primary Schwann Cell Cultures
    16. Primary Dissociated Astrocyte and Neuron Co-culture
    17. Cerebellar Slice Cultures
    18. Hippocampal Slice Cultures 299
    19. Molecular Substrates for Growing Neurons in Culture 313
    20. Guidance and Outgrowth Assays for Embryonic Thalamic Axons 329
    21. Detection of Cell Death in Neuronal Cultures 343
    22. Live Imaging of Neural Cell Functions
    23. Tissue Culture Procedures and Tips
    Index