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Thiol redox transitions in cell signaling. Part A, Chemistry and biochemistry of low molecular weight and protein thiols / volume editor, Enrique Cadenas, Lester Packer. — Amsterdam : Elsevier Academic Press, c2010. – (58.17435/C719/v.473) |
Contents
CONTENTS
Contributors
Preface
Volumes in Series
1. Changing Paradigms in Thiology: From Antioxidant Defense Toward Redox Regulation
1. Introduction
2. Early Concepts, Misconceptions, Unsettled Battles, and Persistent Confusion
3. The Discovery of "Natural" Free Radicals
4. Love Affairs Between Hydroperoxides and Thiolates or Selenolates
5. Toward Regulatory Circuits with Puzzle Stones from Redox Biochemistry
6. Conclusions and Perspectives
References
2. Mass Spectrometry-Based Methods for the Determination of Sulfur and Related Metabolite Concentrations in Cell Extracts 41
1. Introduction
2. Analytical Methods for the Determination of Sulfur and Amino Acid Metabolites
3. Procedures
4. Absolute LC-ESI-MS/MS Quantification of Thiol and Amino Acid Metabolites in Yeast Extracts
5. Qualitative and Quantitative Determination of Thiol and Amino Acid Metabolites in Yeast Extracts by Using an LTQ-Orbitrap Mass Spectrometer
6. Discussion
7. Summary
Acknowledgments
References
3. Use of Dimedone-Based Chemical Probes for Sulfenic Acid Detection: Evaluation of Conditions Affecting Probe Incorporation into Redox-Sensitive Proteins 77
1. Introduction
2. Materials
3. Methods
4. Summary
Acknowledgments
References
4. Use of Dimedone-Based Chemical Probes for Sulfenic Acid Detection: Methods to Visualize and Identify Labeled Proteins
1. Introduction
2. Biotin-Based Affinity Capture to Identify Proteins Containing Cysteine Sulfenic Acids
3. Detection Methods to Identify Oxidized Proteins and Cysteines
4. Summary
Acknowledgments
References
5. Formation and Reactions of Sulfenic Acid in Human Serum Albumin
1. Introduction
2. Preparation of Albumin Solutions
3. Preparation of Oxidized Albumin
4. Detection of Albumin Sulfenic Acid
5. Quantification of Albumin Sulfenic Acid Using Thionitrobenzoate (TNB)
6. Reactivity of Sulfenic Acid
7. Detection of Albumin Sulfinic Acid
8. Conclusions
Acknowledgments
References
6. Determination of GSH, GSSG, and GSNO Using HPLC with Electrochemical Detection
1. Introduction
2. Methods
3. Summary
Acknowledgments
References
7. Measurement of Mixed Disulfides Including Glutathionylated Proteins
1. Introduction
2. Chemical Quantification of PSSX, PSSG, and PSSC
3. Visualization of PSSG by Western Blot
Acknowledgments
References
8. Detection and Quantification of Protein Disulfides in Biological Tissues: A Fluorescence-Based Proteomic Approach
1. Introduction
2. Material and Methods
3. Results
4. Discussion
Acknowledgments
References
9. Measurement and Identification of S-Glutathiolated Proteins 179
1. Introduction
2. Chemical Methods for the Measurement of Glutathiolated Proteins
3. Detection of Glutathiolated Proteins by ESI/MS
4. Identification of S-Glutathiolated Proteins in Cells and Tissues
5. Conclusions
Acknowledgment
References
10. Proteome Screens for Cys Residues Oxidation: The Redoxome 199
1. Introduction
2. General Considerations
3. Overview of the Different Methods
4. Results and Discussion
5. Conclusions
Acknowledgments
References
11. Identification by MS/MS of Disulfides Produced by a Functional Redox Transition
1. Introduction
2. MS/MS Identification of Redox-Switches in Protein
3. Analytical Procedure
4. Discussion
References
12. Mass Spectrometry Approaches for the Redox Characterization of Protein Cysteine Residues: The Case of the Transcription Factor Pax-8
1. Introduction
2. Materials and Methods
3. Results
4. Conclusions and Future Perspectives
Acknowledgments
References
13. A Simple Method to Systematically Study Oxidatively Modified Proteins in Biological Samples and Its Applications 251
1. Introduction
2. Materials
3. Methods
4. Discussion
Acknowledgment
References
14. Direct and Indirect Detection Methods for the Analysis of S-Nitrosylated Peptides and Proteins
1. Introduction
2. Indirect Detection of S-Nitrosylated Proteins: His-tag Switch
3. Direct Analysis of S-Nitrosytation by MS
4. Conclusions
References
15. A Rapid Approach for the Detection, Quantification, and Discovery of Novel Sulfenic Acid or S-Nitrosothiol Modified Proteins Using a Biotin-Switch Method
1. Introduction
2. Overview of Analytical Strategy
3. Conclusions
Acknowledgments
References
16. Protein Adducts of Aldehydic Lipid Peroxidation Products: Identification and Characterization of Protein Adducts Using an Aldehyde/Keto-Reactive Probe in, Combination with Mass Spectrometry
1. Introduction
2. Modification of Proteins by Aldehydic Lipid Peroxidation Products
3. Redox Proteomics of Protein Targets of Reactive Lipid Peroxidation Products
4. Mass Spectrometry-Based Approaches for the Identification and Characterization of Protein Adducts of Aldehydic Lipid Peroxidation Products
5. Experimental Strategy of Using an Aldehyde/Keto-Reactive Probe for the Targeted Analysis of Protein Adducts of Aldehydic Lipid Peroxidation Products
6. Applications of the ARP-Labeling Strategy
7. Interpretation of MS/MS Spectra of Protein Adducts and the Use of Diagnostic Marker Ions
8. Conclusion
Acknowledgment
References
Author Index
Subject Index