A manual for primary human cell culture / series editor, Jan-Thorsten Schantz. — 2nd ed. — Hackensack, NJ ; London : World Scientific, 2010. – (58.221/M294/2nd ed.)

Contents

    Contents
    
    Preface
    List of Figures and Tables
    A Principles of Biosafety
     A.1 Categories of Biosafety
     A.2 Biohazard Materials
     A.3 Recommended Work Practices
     A.4 General Hygiene
     A.5 Handling Biological Specimens
    B Introduction to the Cell Culture Laboratory
     B.1 The Laminar Flow Workbench -- Biosafety Cabinet
     B.2 The Centrifuge
     B.3 The Waterbath
     B.4 The Sink/Wet Space
     B.5 The Autoclave
     B.6 The Refrigerator/Freezer
     B.7 The Incubator
     B.8 The Microscope
    C Protocols for Human Cell Culture
     C.1 Cell Isolation
     C.2 Mesenchymal Cells
     C.3. Epithelial Cells
     C.4 Bone Marrow Derived Cells
     C.5 Stem Cells
    D Reagents and Techniques for Cell Culture
     D.1 Selection of Cell Culture Media
     D.2 Cell Culture Reagents and Media Supplements
     D.3 Cell Dissociation Products
    E Techniques for Manipulating Cell and Studying Cellular Behaviour
     E.1 Cryopreservation and Thawing
     E.2 Transfection
     E.3 Quantitative Cell Proliferation Assays
     E.4 Assessment of Gene Expression
    F Troubleshooting
    Suggested Readings
    Abbreviations
    Index