Molecular biotechnology : principles and applications of recombinant DNA / Bernard R. Glick, Jack J. Pasternak, Cheryl L. Patten. — 4th ed. — Washington, D.C. : ASM Press, c2010. – (58.21/G559/4th ed.)

Contents

    Contents
    
    Preface
    Preface to the First Edition
    PART I FUNDAMENTALS OF MOLECULAR BIOTECHNOLOGY
    CHAPTER 1 The Development of Molecular Biotechnology 3
    CHAPTER 2 DNA， RNA， and Protein Synthesis 14
    CHAPTER 3 Recombinant DNA Technology 47
    CHAPTER 4 Chemical Synthesis， Amplification， and Sequencing of DNA 98
    CHAPTER 5 Bioinformatics， Genomics， and Proteomics 146
    CHAPTER 6 Manipulation of Gene Expression in Prokaryotes 195
    CHAPTER 7 Heterologous Protein Production in Eukaryotic Cells 240
    CHAPTER 8 Directed Mutagenesis and Protein Engineering 290
    PART II MOLECULAR BIOTECHNOLOGY OF MICROBIAL SYSTEMS 331
    CHAPTER 9 Molecular Diagnostics 333
    CHAPTER 10 Protein Therapeutics 379
    CHAPTER 11 Nucleic Acids as Therapeutic Agents 426
    CHAPTER 12 Vaccines 459
    CHAPTER 13 Synthesis of Commercial Products by Recombinant Microorganisms 501
    CHAPTER 14 Bioremediation and Biomass Utilization 551
    CHAPTER 15 Plant Growth-Promoting Bacteria 599
    CHAPTER 16 Microbial Insecticides 652
    CHAPTER 17 Large-Scale Production of Proteins from Recombinant Microorganisms
    PART III MOLECULAR BIOTECHNOLOGY OF EUKARYOTIC SYSTEMS 723
    CHAPTER 18 Genetic Engineering of Plants: Methodology 725
    CHAPTER 19 Engineering Plants To Overcome Biotic and Abiotic Stress 759
    CHAPTER 20 Engineering Plant Quality and Proteins 803
    CHAPTER 21 Transgenic Animals 845
    PART IV MOLECULAR BIOTECHNOLOGY AND SOCIETY 895
    CHAPTER 22 Regulating the Use of Biotechnology 897
    CHAPTER 23 Societal Issues in Biotechnology 923