Antimicrobial peptides : methods and protocols / edited by Andrea Giuliani, Andrea C. Rinaldi. — Totowa, N.J. : Humana, c2010. – (58.17/M592/v.618) |
Contents
Contents
Contributors
SECTION I: ISOLATION, PURIFICATION, DESIGN, AND SYNTHESIS OF ANTIMICROBIAL PEPTIDES
1. Antimicrobial Peptides in Frog Skin Secretions
2. Purification of Antimicrobial Peptides from Human Skin
3. Strategies for the Isolation and Characterization of Antibacterial Lantibiotics
4. Expression and Purification of Recombinant α-Defensins and α-Defensin Precursors in Escherichia coli
5. Production of Recombinant Antimicrobial Peptides in Bacteria
6. Methods for Building Quantitative Structure-Activity Relationship (QSAR) Descriptors and Predictive Models for Computer-Aided Design of Antimicrobial Peptides
7. Synthesis and Thermodynamic Characterization of Small Cyclic Antimicrobial Arginine and Tryptophan-Rich Peptides with Selectivity for Gram-Negative Bacteria
8. Synthesis of Antimicrobial Peptides Using the SPOT Technique 111
9. High-Throughput Screening for Antimicrobial Peptides Using the SPOT Technique
SECTION II: ANALYSIS, PROPERTIES, AND MECHANISMS OF ANTIMICROBIAL PEPTIDES
10. Antimicrobial Peptides: The LPS Connection
11. Binding and Permeabilization of Model Membranes by Amphipathic Peptides
12. Comparing Bacterial Membrane Interactions of Antimicrobial Peptides and Their Mimics
13. Dynamic Transitions of Membrane-Active Peptides 183
14. Solid-State NMR Investigations of Membrane-Associated Antimicrobial Peptides 209
15. Use of Atomic Force Microscopy as a Tool to Understand the Action of Antimicrobial Peptides on Bacteria 235
16. Fluorescence and Electron Microscopy Methods for Exploring Antimicrobial Peptides Mode(s) of Action
17. Molecular Simulations of Antimicrobial Peptides
18. Computer-Based Analysis, Visualization, and Interpretation of Antimicrobial Peptide Activities 287
SECTION III: ANTIMICROBIAL PEPTIDES AS THERAPEUTIC AGENTS
19. Potential Therapeutic Application of Host Defense Peptides 303
20. Therapeutic Potential of HDPs as Immunomodulatory Agents 329
21. Assay Systems for Measurement of Anti-inflammatory Activity 349
22. Ex Vivo Skin Infection Model 359
23. Measuring Antimicrobial Peptide Activity on Epithelial Surfaces in Cell Culture 371
24. Antimicrobial and Antibiofilm Activity of Quorum Sensing Peptides and Peptide Analogues Against Oral Biofilm Bacteria 383
25. Characterization of the Leishmanicidal Activity of Antimicrobial Peptides 393
Subject Index 421