Histology protocols / edited by Tim D. Hewitson, Ian A. Darby. — [Totowa, N.J.] : Humana, c2010. – (58.17/M592/v.611)

Contents

    Contents
    
    Preface
    Contributors
    PART I: TISSUE PROCESSING
    1. Tissue Preparation for Histochemistry: Fixation， Embedding， and Antigen Retrieval for Light Microscopy
    2. An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue
    3. Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus
    PART II: STAINING TECHNIQUES
    4. Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells
    5. Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems
    6. Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections.
    7. High-Pressure Freezing， Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy
    8. Lectin Histochemistry for Light and Electron Microscopy
    9. Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain
    10. Special Stains for Extracellular Matrix
    11. Active Staining of Mouse Embryos for Magnetic Resonance Microscopy
    12. Immunohistochemical Detection of Tumour Hypoxia
    13. In Situ Localization of Apoptosis Using TUNEI
    PART III: IMAGING TECHNIQUES
    14. Use of Confocal Microscopy for Three-dimensional Imaging of Neurons in the Spinal Cord
    15. High-Resolution Confocal Imaging in Tissue
    16. Software-Based Stacking Techniques to Enhance depth of Field and dynamic Range in Digital Photomicrography
    17. Image Analysis and Quantitative Morphology
    Subject Index