Rat genomics : methods and protocols / edited by Ignacio Anegon. — [Totowa, N.J.] : Humana Press, c2010. – (58.17/M592/v.597)

Contents

    Contents
    
    Preface
    Contributors
    1 The Rat: A Model Used in Biomedical Research
    2 Genetic Mapping and Positional Cloning
    3 Sequencing of the Rat Genome and Databases
    4 Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators)
    5 Inducible and Conditional Promoter Systems to Generate Transgenic Animals
    6 Generation of Transgenic Rats by Microinjection of Short DNA Fragments
    7 Generation of Transgenic Rats Using YAC and BAC DNA Constructs
    8 The Use of Lentiviral Vectors to Obtain Transgenic Rats 109
    9 Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA
    10 Procedures for Somatic Cell Nuclear Transfer in the Rat 137
    11 ENU Mutagenesis to Generate Genetically Modified Rat Models 151
    12 Establishment of Embryonic Stem Cells from Rat Blastocysts 169
    13 Derivation， Culture， and In vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts 179
    14 Interference RNA for In vivo Knock-Down of Gene Expression or Genome-Wide Screening Using shRNA 189
    15 Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases
    16 Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics
    17 Generation of Congenic and Consomic Rat Strains
    18 Generation of Rat "Supersonic" Congenic/Conplastic Strains Using Superovulation and Embryo Transfer 267
    19 Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms 277
    20 A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites
    21 Cryopreservation and Orthotopic Transplantation of Rat Ovaries 301
    22 Techniques for In Vitro and In Vivo Fertilization in the Rat
    23 Rat Strain Repositories
    24 Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases
    25 Rat Genomics Applied to Psychiatric Research
    26 Genomics Studies of Immune-Mediated Diseases Using the BN-LEW Rat Model
    27 Rat Models of Cardiovascular Diseases 403
    28 Use of Rat Genomics for Investigating the Metabolic Syndrome
    29 Genomic Research in Rat Models of Kidney Disease
    30 Cancer Research in Rat Models
    Index