Drug-DNA interaction protocols / edited by Keith R. Fox. — 2nd ed. — Totowa, N.J. : Humana Press, c2010. – (58.17/M592/v.613)

Contents

    Contents
    
    Preface
    Contributors
    1 Quantitative Analysis of Small Molecule-Nucleic Acid Interactions with a Biosensor Surface and Surface Plasmon Resonance Detection
    2 Thermal Melting Studies of Ligand DNA Interactions
    3 Circular and Linear Dichroism of Drug-DNA Systems
    4 Drug Binding to DNA-RNA Hybrid Structures
    5 Quantification" of Binding Data Using Capillary Electrophoresis 71
    6 Determination of Equilibrium Association Constants of Ligand-DNA Complexes by Electrospray Mass Spectrometry 89
    7 Detection of Adriamycin-DNA Adducts by Accelerator Mass Spectrometry 103
    8 Molecular Modelling Methods to Quantitate Drug-DNA Interactions 119
    9 Application of Anomalous Diffraction Methods to the Study of DNA and DNA-Complexes 133
    10 DNase I Footprinting 153
    11 Methods to Characterize the Effect of DNA-Modifying Compounds on Nucleosomal DNA 173
    12 REPSA: Combinatorial Approach for Identifying Preferred Drug-DNA Binding Sequences
    13 In vitro Transcription Assay for Resolution of Drug-DNA Interactions at Defined DNA Sequences 207
    14 In vitro Footprinting of Promoter Regions Within Supercoiled Plasmid DNA
    15 Topoisomerase I-Mediated DNA Relaxation as a Tool to Study Intercalation of Small Molecules into Supercoiled DNA 235
    16 A High-Throughput Assay for DNA Topoisomerases and Other Enzymes， Based on DNA Triplex Formation 257
    17 Measurement of DNA Interstrand Crosslinking in Individual Cells Using the Single Cell Gel Electrophoresis (Comet) Assay 267
    18 Measurement of DNA Interstrand Crosslinking in Naked DNA Using Gel-Based Methods 283
    19 An Evaluation Cascade for G-Quadruplex Telomere Targeting Agents in Human Cancer Cells 303
    Index