Ligand-macromolecular interactions in drug discovery : methods and protocols / edited by Ana Cecilia A. Roque. — New York : Humana Press, c2010. – (58.17/M592/v.572)

Contents

    Contents
    
    Preface
    Contributors
    PART I REVIEWS
    1 An Historical Overview of Drug Discovery
    2 Ligand Macromolecule Interactions: Theoretical Principles of Molecular Recognition
    3 X-Ray Crystallography in Drug Discovery
    4 Virtual Screening of Compound Libraries
    PART II PROTOCOLS
    5 Combinatorial Chemistry and the Synthesis of Compound Libraries
    6 Ligand-Based Nuclear Magnetic Resonance Screening Techniques
    7 Isothermal Titration Calorimetry and Differential Scanning Calorimetry
    8 Adaptive Combinatorial Design of Focused Compound Libraries
    9 Chemical Microarrays: A New Tool for Discovery Enzyme Inhibitors
    10 Fluorescence Polarization and Time-Resolved Fluorescence Resonance Energy Transfer Techniques for PI3K Assays
    11 Small Molecule Protein Interaction Profiling with Functional Protein Microarrays
    12 Capillary Electrophoresis in Drug Discovery
    13 SPR in Drug Discovery: Searching Bioactive Compounds in Plant Extracts
    14 Application of Frontal Affinity Chromatography with Mass Spectrometry (FAC-MS) for Stereospecific Ligand-Macromolecule Interaction， Detection and Screening 219
    15 GPC Spin Column HPLC-ESI-MS Methods for Screening Drugs Noncovalently Bound to Proteins
    16 A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening
    17 A Natural Products Approach to Drug Discovery: Probing Modes of Action of Antitumor Agents by Genome-Scale cDNA Library Screening 261
    18 Ligand-Macromolecule Interactions in Live Cells by Fluorescence Correlation Spectroscopy
    Index