Free radicals and antioxidant protocols / edited by Rao M. Uppu ... [et al.]. — 2nd ed. — [Totowa, N.J.] : Humana Press, c2010. – (58.17/M592/v.610)

Contents

    Contents
    
    Preface
    Contributors
    SECTION I REACTIVE OXYGEN AND NITROGEN SPECIES 1
    1. In Vivo Imaging of Free Radicals and Oxygen 3
    2. In Vivo Measurement of Tissue Oxygen Using Electron Paramagnetic Resonance Spectroscopy with Oxygen-Sensitive Paramagnetic Particle， Lithium Phthalocyanine
    3. Measurement of Plasma Nitrite by Chemiluminescence
    4. Determination of Glutathione， Mitochondrial Transmembrane Potential， and Cytotoxicity in H9c2 Cardiomyoblasts Exposed to Reactive Oxygen and Nitrogen Species
    SECTION II NATURAL AND SYNTHETIC ANTIOXIDANTS 63
    5. Phenolic Acids and Flavonoids: Occurrence and Analytical Methods 65
    6. Design， Synthesis， and Action of Antiatherogenic Antioxidants 91
    7. Preparation of Drug-Loaded Polymeric Nanoparticles and Evaluation of the Antioxidant Activity Against Lipid Peroxidation 109
    8. Nanoparticle and Iron Chelators as a Potential Novel Alzheimer Therapy 123
    9. Synthesis and Characterization of Polymer Nanocarriers for the Targeted Delivery of Therapeutic Enzymes
    10. Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo 165
    SECTION III CELLULAR OXIDATIVE STRESS 181
    11. The Generation of Stable Oxidative Stress-Resistant Phenotypes in Chinese Hamster Fibroblasts Chronically Exposed to Hydrogen Peroxide or Hyperoxia
    12. A Simple Method for Effective and Safe Removal of Membrane Cholesterol from Lipid Rafts in Vascular Endothelial Cells: Implications in Oxidant-Mediated Lipid Signaling 201
    13. Superoxide Dismutase - A Target for Gene Therapeutic Approach to Reduce Oxidative Stress in Erectile Dysfunction 213
    14. Assessing the Reductive Capacity of Cells by Measuring the Recycling of Ascorbic and Lipoic Acids 229
    15. Biomarkers of Oxidative Stress: Methods and Measures of Oxidative DNA Damage (COMET Assay) and Telomere Shortening 245
    16. Simultaneous Analysis of Expression of Multiple Redox-Sensitive and Apoptotic Genes in Hypothalamic Neurons Exposed to Cholesterol Secoaldehyde 263
    17. Redox Homeostasis and Cellular Stress Response in Aging and Neurodegeneration 285
    18. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Lung for Pulmonary Hypertension 309
    SECTION IV DNA OXIDATION， OXIDATIVE LIPIDOMICS， AND BIOMARKERS 323
    19. A General Method for Quantifying Sequence Effects on Nucleobase Oxidation in DNA 325
    20. Analysis of Urinary 8-oxo-7，8-dihydro-2'-deoxyguanosine by Liquid Chromatography-Tandem Mass Spectrometry 341
    21. Oxidative Lipidomics of Apoptosis: Quantitative Assessment of Phospholipid Hydroperoxides in Cells and Tissues 353
    22. Simultaneous Analysis of Multiple Lipid Oxidation Products In Vivo by Liquid Chromatographic-Mass Spectrometry (LC-MS) 375
    23. Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation， Purification， and Characterization of Phosphatidylinositol Peroxides 387
    24. Oxidized Low-Density Lipoprotein
    25. Detection and Localization of Markers of Oxidative Stress by In Situ Methods: Application in the Study of Alzheimer Disease
    26. Enzyme Immunoassay of Isoprostanes
    27. Application of Membrane Extraction with Sorbent Interface for Breath Analysis
    Subject Index